The role of acidic proteins from cytoplasmic fractions of Krebs II ascites cells for efficient translation.

Zeitschrift fur Naturforschung. Section C, Biosciences ·Vol. 34 ·No. 1-2 ·1979-00-00 ·Pages 64-75

Setyono B, Schmid HP, Köhler K

Abstract

Acidic proteins with affinity to RNA from cytoplasmic fraction of Krebs II Ascites cells were isolated by means of affinity chromatography on RNA-Sepharose CN-Br-columns. Stepwise elution with 350 mM [K+] and 1000 mM [K+] removed two fractions of proteins both of which are required for the formation of 40S- or 80S-initiation complexes and for efficient translation.

MeSH Terms

Animals Carcinoma, Krebs 2/metabolism Cytoplasm/metabolism DNA, Neoplasm/metabolism Haplorhini HeLa Cells/metabolism Kinetics Mice Mice, Inbred BALB C Peptide Chain Initiation, Translational Peptide Elongation Factors/isolation & purification,metabolism Peptide Initiation Factors/isolation & purification,metabolism Polyribosomes/metabolism Protein Biosynthesis RNA, Messenger/metabolism

Chemicals

DNA, Neoplasm Peptide Elongation Factors Peptide Initiation Factors RNA, Messenger

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Setyono B

Schmid H P

Köhler K

Article Info

Journal

Zeitschrift fur Naturforschung. Section C, Biosciences

Abbr.

Z Naturforsch C Biosci

ISSN

0341-0382

DOI

10.1515/znc-1979-1-215

Published

1979-00-00

Pages

64-75

Language

English

Region

Germany

NLM ID

7801143

Subset

External Links

PubMed source DOI: 10.1515/znc-1979-1-215

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The role of acidic proteins from cytoplasmic fractions of Krebs II ascites cells for efficient translation.

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