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PMID: 15634698 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis.

Development (Cambridge, England) ·Vol. 132 ·No. 3 ·2005-02-00 ·Pages 553-63

Brown DD, Martz SN, Binder O, Goetz SC, Price BM, Smith JC, Conlon FL

Abstract

Members of the T-box family of proteins play a fundamental role in patterning the developing vertebrate heart; however, the precise cellular requirements for any one family member and the mechanism by which individual T-box genes function remains largely unknown. In this study, we have investigated the cellular and molecular relationship between two T-box genes, Tbx5 and Tbx20. We demonstrate that blocking Tbx5 or Tbx20 produces phenotypes that display a high degree of similarity, as judged by overall gross morphology, molecular marker analysis and cardiac physiology, implying that the two genes are required for and have non-redundant functions in early heart development. In addition, we demonstrate that although co-expressed, Tbx5 and Tbx20 are not dependent on the expression of one another, but rather have a synergistic role during early heart development. Consistent with this proposal, we show that TBX5 and TBX20 can physically interact and map the interaction domains, and we show a cellular interaction for the two proteins in cardiac development, thus providing the first evidence for direct interaction between members of the T-box gene family.

MeSH Terms
Animals Cell Count Cell Line Cell Nucleus/metabolism Embryo, Nonmammalian/embryology,metabolism Gene Expression Regulation, Developmental Heart/embryology Humans Mice Morphogenesis Myocardium/cytology,metabolism Protein Binding T-Box Domain Proteins/genetics,metabolism Transcription, Genetic/genetics Xenopus laevis/embryology,genetics,metabolism
Chemicals
T-Box Domain Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Brown Daniel D
Department of Genetics, Fordham Hall, UNC-Chapel Hill, Chapel Hill, NC 27599-3280, USA.
Martz Shauna N
Binder Olav
Goetz Sarah C
Price Brenda M J
Smith Jim C
Conlon Frank L
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Article Info
Journal
Development (Cambridge, England)
Abbr.
Development
ISSN
0950-1991
DOI
10.1242/dev.01596
Published
2005-02-00
Epub
2005-00-05
Pages
553-63
Language
English
Region
England
NLM ID
8701744
PMCID
PMC1635804
Subset
IM
Grants
NHLBI NIH HHS · R01 HL075256 · United States
External Links
PubMed source DOI: 10.1242/dev.01596 PMC Full Text View journal details
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