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PMID: 15665075 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The diversity of dolichol-linked precursors to Asn-linked glycans likely results from secondary loss of sets of glycosyltransferases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 5 ·2005-02-01 ·Pages 1548-53

Samuelson J, Banerjee S, Magnelli P, Cui J, Kelleher DJ, Gilmore R, Robbins PW

Abstract

The vast majority of eukaryotes (fungi, plants, animals, slime mold, and euglena) synthesize Asn-linked glycans (Alg) by means of a lipid-linked precursor dolichol-PP-GlcNAc2Man9Glc3. Knowledge of this pathway is important because defects in the glycosyltransferases (Alg1-Alg12 and others not yet identified), which make dolichol-PP-glycans, lead to numerous congenital disorders of glycosylation. Here we used bioinformatic and experimental methods to characterize Alg glycosyltransferases and dolichol-PP-glycans of diverse protists, including many human pathogens, with the following major conclusions. First, it is demonstrated that common ancestry is a useful method of predicting the Alg glycosyltransferase inventory of each eukaryote. Second, in the vast majority of cases, this inventory accurately predicts the dolichol-PP-glycans observed. Third, Alg glycosyltransferases are missing in sets from each organism (e.g., all of the glycosyltransferases that add glucose and mannose are absent from Giardia and Plasmodium). Fourth, dolichol-PP-GlcNAc2Man5 (present in Entamoeba and Trichomonas) and dolichol-PP- and N-linked GlcNAc2 (present in Giardia) have not been identified previously in wild-type organisms. Finally, the present diversity of protist and fungal dolichol-PP-linked glycans appears to result from secondary loss of glycosyltransferases from a common ancestor that contained the complete set of Alg glycosyltransferases.

MeSH Terms
Animals Asparagine Bacteria/enzymology,genetics Computational Biology Dolichols/metabolism Evolution, Molecular Genetic Variation Glycopeptides/biosynthesis Glycosyltransferases/genetics,metabolism Humans Polysaccharides/chemistry Species Specificity
Chemicals
Dolichols Glycopeptides Polysaccharides Asparagine Glycosyltransferases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Samuelson John
Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany Street, Boston, MA 02118-2932, USA. [email protected]
Banerjee Sulagna
Magnelli Paula
Cui Jike
Kelleher Daniel J
Gilmore Reid
Robbins Phillips W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0409460102
Email
[email protected]
Published
2005-02-01
Epub
2005-00-21
Pages
1548-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC545090
Subset
IM
Grants
NIGMS NIH HHS · GM31318 · United States
NIGMS NIH HHS · R01 GM043768 · United States
NIGMS NIH HHS · R01 GM031318 · United States
NIAID NIH HHS · AI44070 · United States
NIGMS NIH HHS · GM43768 · United States
NIAID NIH HHS · AI48082 · United States
NIAID NIH HHS · R01 AI048082 · United States
NIAID NIH HHS · R01 AI044070 · United States
External Links
PubMed source DOI: 10.1073/pnas.0409460102 PMC Full Text View journal details
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