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PMID: 15673612 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rab8 regulates the actin-based movement of melanosomes.

Molecular biology of the cell ·Vol. 16 ·No. 4 ·2005-04-00 ·Pages 1640-50

Chabrillat ML, Wilhelm C, Wasmeier C, Sviderskaya EV, Louvard D, Coudrier E

Abstract

Rab GTPases have been implicated in the regulation of specific microtubule- and actin-based motor proteins. We devised an in vitro motility assay reconstituting the movement of melanosomes on actin bundles in the presence of ATP to investigate the role of Rab proteins in the actin-dependent movement of melanosomes. Using this assay, we confirmed that Rab27 is required for the actin-dependent movement of melanosomes, and we showed that a second Rab protein, Rab8, also regulates this movement. Rab8 was partially associated with mature melanosomes. Expression of Rab8Q67L perturbed the cellular distribution and increased the frequency of microtubule-independent movement of melanosomes in vivo. Furthermore, anti-Rab8 antibodies decreased the number of melanosomes moving in vitro on actin bundles, whereas melanosomes isolated from cells expressing Rab8Q67L exhibited 70% more movements than wild-type melanosomes. Together, our observations suggest that Rab8 is involved in regulating the actin-dependent movement of melanosomes.

MeSH Terms
Actins/metabolism Animals Biological Transport Cell Line Humans Melanosomes/metabolism Mice Mutation/genetics rab GTP-Binding Proteins/genetics,metabolism rab27 GTP-Binding Proteins
Chemicals
Actins rab27 GTP-Binding Proteins RAB27A protein, human RAB8A protein, human Rab27a protein, mouse rab GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chabrillat Marion L
Unité de Morphogenèse et Signalisation Cellulaires, Institut Curie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 144, Paris Cedex, France.
Wilhelm Claire
Wasmeier Christina
Sviderskaya Elena V
Louvard Daniel
Coudrier Evelyne
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-09-0770
Published
2005-04-00
Epub
2005-00-26
Pages
1640-50
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1073648
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e04-09-0770 PMC Full Text View journal details
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