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PMID: 15693946 Published · ppublish English Evaluation Study Journal Article

Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails.

Genome biology ·Vol. 6 ·No. 2 ·2005-00-00 ·Pages R17

Kunitomo H, Uesugi H, Kohara Y, Iino Y

Abstract

It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes.

MeSH Terms
Animals Caenorhabditis elegans/embryology,genetics,metabolism Cilia Gene Expression Profiling Genes, Reporter Helminth Proteins/genetics Immunoprecipitation/methods Neurons, Afferent/metabolism Oligonucleotide Array Sequence Analysis Oligopeptides Peptides/genetics,immunology Poly(A)-Binding Proteins/genetics,isolation & purification RNA, Messenger/isolation & purification,metabolism Recombinant Fusion Proteins/analysis
Chemicals
Helminth Proteins Oligopeptides Peptides Poly(A)-Binding Proteins RNA, Messenger Recombinant Fusion Proteins che-2 protein, C elegans FLAG peptide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kunitomo Hirofumi
Molecular Genetics Research Laboratory, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. [email protected]
Uesugi Hiroko
Kohara Yuji
Iino Yuichi
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Article Info
Journal
Genome biology
Abbr.
Genome Biol
ISSN
1474-760X
DOI
10.1186/gb-2005-6-2-r17
Email
[email protected]
Published
2005-00-00
Epub
2005-00-31
Pages
R17
Language
English
Region
England
NLM ID
100960660
PMCID
PMC551537
Subset
IM
External Links
PubMed source DOI: 10.1186/gb-2005-6-2-r17 PMC Full Text View journal details
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