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PMID: 15705958 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length.

The Plant cell ·Vol. 17 ·No. 3 ·2005-03-00 ·Pages 776-90

Tanabe S, Ashikari M, Fujioka S, Takatsuto S, Yoshida S, Yano M, Yoshimura A, Kitano H, Matsuoka M, Fujisawa Y, Kato H, Iwasaki Y

Abstract

We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, the D11 gene was isolated by a map-based cloning method. The gene was found to encode a novel cytochrome P450 (CYP724B1), which showed homology to enzymes involved in brassinosteroid (BR) biosynthesis. The dwarf phenotype of d11 mutants was restored by the application of the brassinolide (BL). Compared with wild-type plants, the aberrant D11 mRNA accumulated at higher levels in d11 mutants and was dramatically reduced by treatment with BL, implying that the gene is feedback-regulated by BL. Precise determination of the defective step(s) in BR synthesis in d11 mutants proved intractable because of tissue specificity and the complex control of BR accumulation in plants. However, 6-deoxotyphasterol (6-DeoxoTY) and typhasterol (TY), but not any upstream intermediates before these compounds, effectively restored BR response in d11 mutants in a lamina joint bending assay. Multiple lines of evidence together suggest that the D11/CYP724B1 gene plays a role in BR synthesis and may be involved in the supply of 6-DeoxoTY and TY in the BR biosynthesis network in rice.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Cytochrome P-450 Enzyme System/genetics,metabolism DNA, Plant/genetics Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Light Molecular Sequence Data Mutation Oryza/genetics,growth & development,metabolism,radiation effects Phenotype Phylogeny Plant Growth Regulators/biosynthesis Seeds/growth & development Sequence Homology, Amino Acid Steroids, Heterocyclic/metabolism
Chemicals
DNA, Plant Plant Growth Regulators Steroids, Heterocyclic Cytochrome P-450 Enzyme System
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Tanabe Sumiyo
Fukui Prefectural University, Faculty of Bioscience, Kenjyojima, Matsuoka-cho, Yoshida-gun, Fukui 910-1195, Japan.
Ashikari Motoyuki
Fujioka Shozo
Takatsuto Suguru
Yoshida Shigeo
Yano Masahiro
Yoshimura Atsushi
Kitano Hidemi
Matsuoka Makoto
Fujisawa Yukiko
Kato Hisaharu
Iwasaki Yukimoto
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.024950
Published
2005-03-00
Epub
2005-00-10
Pages
776-90
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1069698
Subset
IM
Grants
NCIRD CDC HHS · H23 IP001002 · United States
Databases
GENBANK
AAN60994, AB008097, AB035868, AB066286, AB084385, AB158759, AC006592, AC130732, AF044216, AF318500, AF318501, AP003244, BAA28531, U54770, X16280, X87367
RefSeq
NM_191780
External Links
PubMed source DOI: 10.1105/tpc.104.024950 PMC Full Text View journal details
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