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PMID: 15716313 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

mRNA sequence features that contribute to translational regulation in Arabidopsis.

Nucleic acids research ·Vol. 33 ·No. 3 ·2005-00-00 ·Pages 955-65

Kawaguchi R, Bailey-Serres J

Abstract

DNA microarrays were used to evaluate the regulation of the proportion of individual mRNA species in polysomal complexes in leaves of Arabidopsis thaliana under control growth conditions and following a mild dehydration stress (DS). The analysis determined that the percentage of an individual gene transcript in polysomes (ribosome loading) ranged from over 95 to <5%. DS caused a decrease in ribosome loading from 82 to 72%, with maintained polysome association for over 60% of the mRNAs with an increased abundance. To identify sequence features responsible for translational regulation, ribosome loading values and features of full-length mRNA sequences were compared. mRNAs with extreme length or high GU content in the 5'-untranslated regions (5'-UTRs) were generally poorly translated. Under DS, mRNAs with both a high GC content in the 5'-UTR and long open reading frame showed a significant impairment in ribosome loading. Evaluation of initiation A+1UG codon context revealed distinctions in the frequency of adenine in nucleotides -10 to -1 (especially at -4 and -3) in mRNAs with different ribosome loading values. Notably, the mRNA features that contribute to translational regulation could not fully explain the variation in ribosome loading, indicating that additional factors contribute to translational regulation in Arabidopsis.

MeSH Terms
3' Untranslated Regions 5' Untranslated Regions Arabidopsis/genetics,metabolism Codon Codon, Initiator Gene Expression Profiling Gene Expression Regulation, Plant Oligonucleotide Array Sequence Analysis Open Reading Frames Polyribosomes/metabolism Protein Biosynthesis RNA, Messenger/chemistry RNA, Plant/chemistry Regulatory Sequences, Ribonucleic Acid Ribosomes/metabolism
Chemicals
3' Untranslated Regions 5' Untranslated Regions Codon Codon, Initiator RNA, Messenger RNA, Plant Regulatory Sequences, Ribonucleic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kawaguchi Riki
Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California Riverside, CA 92521, USA.
Bailey-Serres Julia
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gki240
Published
2005-00-00
Epub
2005-00-16
Pages
955-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC549406
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gki240 PMC Full Text View journal details
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