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PMID: 15726663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transdifferentiation of rat hepatocytes into biliary cells after bile duct ligation and toxic biliary injury.

Hepatology (Baltimore, Md.) ·Vol. 41 ·No. 3 ·2005-03-00 ·Pages 535-44

Michalopoulos GK, Barua L, Bowen WC

Abstract

Rats with chimeric livers were generated by using the protocol of injecting hepatocytes from dipeptidyl peptidase IV (DPPIV)-positive donors into retrorsine-treated DPPIV-negative recipients subjected to partial hepatectomy. Rats with established chimeric livers were subjected to bile duct ligation, with or without pretreatment with the biliary toxin methylene diamiline (DAPM). Ductules bearing the donor hepatocyte marker DPPIV were seen at 30 days after bile duct ligation. The frequency of the ductules derived from the donor hepatocytes was dramatically enhanced (36-fold) by the pretreatment with DAPM. In conclusion, our results show that hepatocytes can function as facultative stem cells and rescue the biliary epithelium during repair from injury when its proliferative capacity is being compromised.

MeSH Terms
Aniline Compounds/toxicity Animals Bile Ducts Biliary Tract/drug effects,pathology Cell Differentiation Cell Proliferation Dipeptidyl Peptidase 4/analysis Epithelial Cells/pathology Hepatocytes/pathology Ligation Liver Regeneration Male Rats Rats, Inbred F344
Chemicals
Aniline Compounds Dipeptidyl Peptidase 4 4,4'-diaminodiphenylmethane
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Michalopoulos George K
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. [email protected]
Barua Lindsay
Bowen William C
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Article Info
Journal
Hepatology (Baltimore, Md.)
Abbr.
Hepatology
ISSN
0270-9139
DOI
10.1002/hep.20600
Email
[email protected]
Published
2005-03-00
Pages
535-44
Language
English
Region
United States
NLM ID
8302946
PMCID
PMC1821079
Subset
IM
Grants
NCI NIH HHS · R01 CA035373 · United States
NCI NIH HHS · R01 CA103958 · United States
NCI NIH HHS · CA103958 · United States
NCI NIH HHS · CA35373 · United States
External Links
PubMed source DOI: 10.1002/hep.20600 PMC Full Text View journal details
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