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PMID: 15731097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence.

Infection and immunity ·Vol. 73 ·No. 3 ·2005-03-00 ·Pages 1898-902

Peirs P, Lefèvre P, Boarbi S, Wang XM, Denis O, Braibant M, Pethe K, Locht C, Huygen K, Content J

Abstract

By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding proteins. In a mouse infection model, both mutants were attenuated in virulence, suggesting that M. tuberculosis encounters limiting phosphate concentrations during its intracellular life span.

MeSH Terms
Animals Bacterial Proteins/genetics,metabolism Culture Media Gene Deletion Humans Mice Mice, Inbred BALB C Mice, Inbred C57BL Mycobacterium tuberculosis/genetics,growth & development,metabolism,pathogenicity Phosphate-Binding Proteins/genetics,metabolism Phosphates/metabolism Tuberculosis/microbiology,physiopathology Virulence
Chemicals
Bacterial Proteins Culture Media Phosphate-Binding Proteins Phosphates
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Peirs Priska
Pasteur Institute of Brussels, Engelandstraat 642, B-1180-Brussels, Belgium.
Lefèvre Philippe
Boarbi Samira
Wang Xiao-Ming
Denis Olivier
Braibant Martine
Pethe Kevin
Locht Camille
Huygen Kris
Content Jean
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.73.3.1898-1902.2005
Published
2005-03-00
Pages
1898-902
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1064925
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.73.3.1898-1902.2005 PMC Full Text View journal details
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