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PMID: 15734921 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Crystal structures of alfalfa caffeoyl coenzyme A 3-O-methyltransferase.

Plant physiology ·Vol. 137 ·No. 3 ·2005-03-00 ·Pages 1009-17

Ferrer JL, Zubieta C, Dixon RA, Noel JP

Abstract

Caffeoyl coenzyme A 3-O-methyltransferases (CCoAOMTs) are S-adenosyl-l-methionine-dependent O-methyltransferases (OMTs) involved in lignin biosynthesis. Plant CCoAOMTs belong to a distinct family of OMTs, more closely related to the mammalian catechol OMTs than to other plant OMTs. The crystal structure of alfalfa (Medicago sativa) CCoAOMT in complex with the reaction products S-adenosine-l-homocysteine and feruloyl/sinapoyl CoAs presented here belong to a structurally and mechanistically distinct family of plant small molecule OMTs. These structures provide a new understanding of the substrate preferences and the catalytic mechanism accompanying CCoAOMT-mediated O-methylation of CoA-linked phenylpropanoid substrates.

MeSH Terms
Amino Acid Sequence Binding Sites Crystallography, X-Ray Medicago sativa/enzymology Methyltransferases/chemistry,metabolism Models, Molecular Molecular Sequence Data Protein Conformation Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Methyltransferases caffeoyl-CoA O-methyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ferrer Jean-Luc
Laboratoire de Cristallographie et Cristallogenèse des Protéines, Institut de Biologie Structurale, 38027 Grenoble cedex 1, France.
Zubieta Chloe
Dixon Richard A
Noel Joseph P
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.104.048751
Published
2005-03-00
Epub
2005-00-25
Pages
1009-17
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1065401
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.104.048751 PMC Full Text View journal details
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