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PMID: 15744494 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antisense reduction of thylakoidal ascorbate peroxidase in Arabidopsis enhances paraquat-induced photooxidative stress and nitric oxide-induced cell death.

Planta ·Vol. 221 ·No. 6 ·2005-08-00 ·Pages 757-65

Tarantino D, Vannini C, Bracale M, Campa M, Soave C, Murgia I

Abstract

The production and characterization of Arabidopsis plants containing a transgene in which the Arabidopsis tAPX is inserted in antisense orientation, is described. tAPX activity in these transgenic tAPX plants is around 50% of control level. The tAPX antisense plants are phenotypically indistinguishable from control plants under normal growth conditions; they show, however, enhanced sensitivity to the O2- -generating herbicide, Paraquat. Interestingly, the tAPX antisense plants show enhanced symptoms of damage when cell death is triggered through treatment with the nitric oxide-donor, SNP. These results are in accordance with the ones recently obtained with transgenic plants overexpressing tAPX; altogether, they suggest that tAPX, besides the known ROS scavenging role, is also involved in the fine changes of H2O2 concentration during signaling events.

MeSH Terms
Arabidopsis/enzymology,genetics Arabidopsis Proteins Ascorbate Peroxidases Cell Death/physiology DNA, Antisense Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Herbicides/pharmacology Light Nitric Oxide/physiology Oxidative Stress/physiology Paraquat/pharmacology Peroxidases/genetics,metabolism Phenotype Plants, Genetically Modified Thylakoid Membrane Proteins Thylakoids/enzymology
Chemicals
Arabidopsis Proteins DNA, Antisense Herbicides Thylakoid Membrane Proteins Nitric Oxide Peroxidases APXT protein, Arabidopsis Ascorbate Peroxidases Paraquat
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tarantino Delia
Dipartimento di Biologia, Sezione di Fisiologia e Biochimica delle Piante, Università degli Studi di Milano, via Celoria 26, 20133, Italy.
Vannini Candida
Bracale Marcella
Campa Manuela
Soave Carlo
Murgia Irene
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-005-1485-9
Published
2005-08-00
Epub
2005-00-03
Pages
757-65
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-005-1485-9 View journal details
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