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PMID: 15802291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sensory transduction of pulmonary reactive oxygen species by capsaicin-sensitive vagal lung afferent fibres in rats.

The Journal of physiology ·Vol. 565 ·No. Pt 2 ·2005-06-01 ·Pages 563-78

Ruan T, Lin YS, Lin KS, Kou YR

Abstract

The mechanisms of sensory transduction of pulmonary reactive oxygen species (ROS) by capsaicin-sensitive vagal lung afferent fibres are unclear. To investigate the role of transient receptor potential vanilloid 1 (TRPV1) receptors and P2X purinoceptors in this sensory transduction, we recorded fibre activity (FA) from 132 fibres of this type in 132 anaesthetized and ventilated rats. Airway challenge of aerosolized H2O2 (0, 0.2 and 0.4%) produced a concentration-dependant fibre stimulation. The fibre responses to 0.4% H2O2 were attenuated by dimethylthiourea (a hydroxyl radical (.OH) scavenger; change in fibre activity (DeltaFA), -55+/-9%) or deferoxamine (an iron-chelator that prevents formation of .OH; DeltaFA, -59+/-9%), were prevented by catalase (an enzyme catalysing H2O2; DeltaFA, -96+/-3%) and were unaffected by the vehicle for dimethylthiourea, iron-saturated deferoxamine or heat-inactivated catalase. The fibre responses to 0.4% H2O2 were attenuated by capsazepine (a TRPV1 receptor antagonist; DeltaFA, -39+/-9%) or iso-pyridoxalphosphate-6-azophenyl-2',5'-disulphonate (iso-PPADS, a P2X receptor antagonist; DeltaFA, -51+/-9%), were further reduced by capsazepine and iso-PPADS in combination (DeltaFA, -70+/-13%), and were unaltered by their vehicles. The fibre responses to cigarette smoke (20 ml), an irritant that generates ROS, were attenuated by dimethylthiourea (DeltaFA, -61+/-9%) or capsazepine and iso-PPADS in combination (DeltaFA, -67+/-9%). These results suggest that both the TRPV1 and P2X receptors mediate the sensory transduction of ROS, especially H2O2 and .OH, by capsaicin-sensitive vagal lung afferent fibres.

MeSH Terms
Action Potentials/drug effects,physiology Animals Blood Pressure/drug effects Capsaicin/pharmacology Hydrogen Peroxide/pharmacology Ion Channels/physiology Lung/innervation,metabolism Lung Compliance/drug effects Male Neurons, Afferent/drug effects,physiology Oxidants/pharmacology Rats Rats, Sprague-Dawley Reactive Oxygen Species/metabolism Receptors, Purinergic P2/physiology Smoking Stimulation, Chemical TRPV Cation Channels Vagus Nerve/cytology,drug effects,physiology
Chemicals
Ion Channels Oxidants Reactive Oxygen Species Receptors, Purinergic P2 TRPV Cation Channels Trpv1 protein, rat Hydrogen Peroxide Capsaicin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ruan Ting
Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
Lin You Shuei
Lin Kae-Shin
Kou Yu Ru
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.2005.086181
Published
2005-06-01
Epub
2005-00-31
Pages
563-78
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1464522
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.2005.086181 PMC Full Text View journal details
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