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PMID: 15805477 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Comprehensive interaction map of the Arabidopsis MADS Box transcription factors.

The Plant cell ·Vol. 17 ·No. 5 ·2005-05-00 ·Pages 1424-33

de Folter S, Immink RG, Kieffer M, Parenicová L, Henz SR, Weigel D, Busscher M, Kooiker M, Colombo L, Kater MM, Davies B, Angenent GC

Abstract

Interactions between proteins are essential for their functioning and the biological processes they control. The elucidation of interaction maps based on yeast studies is a first step toward the understanding of molecular networks and provides a framework of proteins that possess the capacity and specificity to interact. Here, we present a comprehensive plant protein-protein interactome map of nearly all members of the Arabidopsis thaliana MADS box transcription factor family. A matrix-based yeast two-hybrid screen of >100 members of this family revealed a collection of specific heterodimers and a few homodimers. Clustering of proteins with similar interaction patterns pinpoints proteins involved in the same developmental program and provides valuable information about the participation of uncharacterized proteins in these programs. Furthermore, a model is proposed that integrates the floral induction and floral organ formation networks based on the interactions between the proteins involved. Heterodimers between flower induction and floral organ identity proteins were observed, which point to (auto)regulatory mechanisms that prevent the activity of flower induction proteins in the flower.

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Chromosome Mapping/methods Dimerization Flowers/genetics,growth & development,metabolism Gene Expression Profiling Gene Expression Regulation, Developmental/genetics Gene Expression Regulation, Plant/genetics Genome, Plant MADS Domain Proteins/genetics,metabolism Macromolecular Substances Phylogeny Proteomics Transcription Factors/genetics,metabolism Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins MADS Domain Proteins Macromolecular Substances Transcription Factors
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
de Folter Stefan
Business Unit Bioscience, Plant Research International, 6700 AA Wageningen, The Netherlands.
Immink Richard G H
Kieffer Martin
Parenicová Lucie
Henz Stefan R
Weigel Detlef
Busscher Marco
Kooiker Maarten
Colombo Lucia
Kater Martin M
Davies Brendan
Angenent Gerco C
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.031831
Published
2005-05-00
Epub
2005-00-01
Pages
1424-33
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1091765
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.031831 PMC Full Text View journal details
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