Abstract
This study determined that the effect of 9-beta-d-arabinofuranosyl-adenine (adenine arabinoside, Ara-A) upon vaccinia virus plaque development in the stable monkey kidney line, LLC-MK(2), was increased approximately 40-fold when an inhibitor of adenosine deaminase (ADA) was added to the tissue culture media along with infective inocula. The concentration of Ara-A required to completely suppress plaque development (total plaque inhibitory concentration(100); TPIC(100)) was greater than 10 mug/ml. However, when ADA activity was inhibited, the TPIC(100) was 0.5 mug/ml or less. Chromatographic assay of arabinosylpurines in the media provided evidence that adenine arabinoside was rapidly deaminated to 9-beta-d-arabinofuranosylhypoxanthine by the cellular monolayers, in the absence of animal serum, and that the rate of deamination, at 5 mug/ml, by the cells was equal to the rate of diffusion of Ara-A across the cellular membrane. The half-life of Ara-A in the media, starting with 5 mug/ml, was 2 to 3 h and shorter at lower concentrations. The study demonstrates the profound effect that an indicator system, acting as an intact biological unit, can have upon a potential antiviral compound.
MeSH Terms
Adenosine Deaminase/physiology
Adenosine Deaminase Inhibitors
Animals
Antiviral Agents/pharmacology
Cell Line
Enzyme Inhibitors/pharmacology
Vaccinia virus/drug effects,enzymology
Vidarabine/pharmacology
Chemicals
Adenosine Deaminase Inhibitors
Antiviral Agents
Enzyme Inhibitors
Adenosine Deaminase
Vidarabine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Connor J D
Sweetman L
Carey S
Stuckey M A
Buchanan R
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18 references, click to expand
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