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PMID: 15852021 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

Target accessibility dictates the potency of human RISC.

Nature structural & molecular biology ·Vol. 12 ·No. 5 ·2005-05-00 ·Pages 469-70

Brown KM, Chu CY, Rana TM

Abstract

In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.

MeSH Terms

Base Sequence Binding Sites Catalysis Cyclin-Dependent Kinase 9/genetics,metabolism HIV Long Terminal Repeat/genetics HeLa Cells Humans Kinetics Molecular Sequence Data Nucleic Acid Conformation RNA, Viral/chemistry,genetics,metabolism RNA-Induced Silencing Complex/metabolism

Chemicals

RNA, Viral RNA-Induced Silencing Complex Cyclin-Dependent Kinase 9

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Brown Kirk M

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.

Chu Chia-Ying

Rana Tariq M

Article Info

Journal

Nature structural & molecular biology

Abbr.

Nat Struct Mol Biol

ISSN

1545-9993

DOI

10.1038/nsmb931

Published

2005-05-00

Epub

2005-00-24

Pages

469-70

Language

English

Region

United States

NLM ID

101186374

Subset

Grants

NIAID NIH HHS · AI 41404 · United States

NIAID NIH HHS · AI 43198 · United States

External Links

PubMed source DOI: 10.1038/nsmb931 View journal details

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Target accessibility dictates the potency of human RISC.

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