Home LiteratureArticle Details
PMID: 15908407 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

responses of mycobacterium tuberculosis to growth in the mouse lung.

Infection and immunity ·Vol. 73 ·No. 6 ·2005-06-00 ·Pages 3754-7

Dubnau E, Chan J, Mohan VP, Smith I

Abstract

Using a promoter trap, we have identified 56 Mycobacterium tuberculosis genes preferentially expressed in the mouse lung. Quantitative real-time PCR showed that RNA levels of several genes were higher from bacteria growing in mouse lungs than from broth cultures. These results support the current hypothesis that Mycobacterium tuberculosis utilizes fatty acids as a carbon source in the mouse lung.

MeSH Terms
Animals Antigens, Bacterial/genetics Bacterial Proteins Drug Resistance, Bacterial Isoniazid/pharmacology Lung/microbiology Mice Mycobacterium tuberculosis/growth & development Oxidoreductases/genetics Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA
Chemicals
Antigens, Bacterial Bacterial Proteins ESAT-6 protein, Mycobacterium tuberculosis Oxidoreductases InhA protein, Mycobacterium Isoniazid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dubnau Eugenie
The Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
Chan John
Mohan V P
Smith Issar
References (20)
20 references, click to expand
  1. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
    Nature. 1998 Jun 11;393(6685):537-44 PMID: 9634230
  2. Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis.
    J Bacteriol. 1996 Mar;178(5):1274-82 PMID: 8631702
  3. Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis.
    Infect Immun. 1999 Apr;67(4):1702-7 PMID: 10085007
  4. Reactivation of latent tuberculosis: variations on the Cornell murine model.
    Infect Immun. 1999 Sep;67(9):4531-8 PMID: 10456896
  5. Immunogenicity of DNA vaccines expressing tuberculosis proteins fused to tissue plasminogen activator signal sequences.
    Infect Immun. 1999 Sep;67(9):4780-6 PMID: 10456931
  6. Biochemical differentiation of Mycobacterium tuberculosis grown in vivo and in vitro.
    J Bacteriol. 1956 Aug;72(2):132-41 PMID: 13366889
  7. Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens.
    Infect Immun. 2000 Jan;68(1):214-20 PMID: 10603390
  8. Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase.
    Nature. 2000 Aug 17;406(6797):735-8 PMID: 10963599
  9. Protection of mice with a tuberculosis subunit vaccine based on a fusion protein of antigen 85b and esat-6.
    Infect Immun. 2001 May;69(5):2773-8 PMID: 11292688
  10. Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from the intracellular pathogen Rhodococcus equi.
    J Bacteriol. 2001 Aug;183(16):4796-805 PMID: 11466283
  11. The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages.
    Mol Microbiol. 2001 Nov;42(3):851-65 PMID: 11722747
  12. Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable for in vivo growth.
    Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3147-52 PMID: 11880648
  13. Mycobacterium tuberculosis genes induced during infection of human macrophages.
    Infect Immun. 2002 Jun;70(6):2787-95 PMID: 12010964
  14. ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response.
    Infect Immun. 2002 Jul;70(7):3371-81 PMID: 12065475
  15. Expression of Th1-mediated immunity in mouse lungs induces a Mycobacterium tuberculosis transcription pattern characteristic of nonreplicating persistence.
    Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):241-6 PMID: 12506197
  16. Transcriptional Adaptation of Mycobacterium tuberculosis within Macrophages: Insights into the Phagosomal Environment.
    J Exp Med. 2003 Sep 1;198(5):693-704 PMID: 12953091
  17. Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients.
    Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14321-6 PMID: 14623960
  18. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis.
    Science. 1994 Jan 14;263(5144):227-30 PMID: 8284673
  19. Mutation of the principal sigma factor causes loss of virulence in a strain of the Mycobacterium tuberculosis complex.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):8036-40 PMID: 7644534
  20. Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis.
    J Infect Dis. 1999 Mar;179(3):637-45 PMID: 9952370
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.73.6.3754-3757.2005
Published
2005-06-00
Pages
3754-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1111836
Subset
IM
Grants
NHLBI NIH HHS · R01 HL064544 · United States
NHLBI NIH HHS · HL 64544 · United States
External Links
PubMed source DOI: 10.1128/IAI.73.6.3754-3757.2005 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2