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PMID: 15923330 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The role of salicylic acid in the induction of cell death in Arabidopsis acd11.

Plant physiology ·Vol. 138 ·No. 2 ·2005-06-00 ·Pages 1037-45

Brodersen P, Malinovsky FG, Hématy K, Newman MA, Mundy J

Abstract

Salicylic acid (SA) is implicated in the induction of programmed cell death (PCD) associated with pathogen defense responses because SA levels increase in response to PCD-inducing infections, and PCD development can be inhibited by expression of salicylate hydroxylase encoded by the bacterial nahG gene. The acd11 mutant of Arabidopsis (Arabidopsis thaliana L. Heynh.) activates PCD and defense responses that are fully suppressed by nahG. To further study the role of SA in PCD induction, we compared phenotypes of acd11/nahG with those of acd11/eds5-1 and acd11/sid2-2 mutants deficient in a putative transporter and isochorismate synthase required for SA biosynthesis. We show that sid2-2 fully suppresses SA accumulation and cell death in acd11, although growth inhibition and premature leaf chlorosis still occur. In addition, application of exogenous SA to acd11/sid2-2 is insufficient to restore cell death. This indicates that isochorismate-derived compounds other than SA are required for induction of PCD in acd11 and that some acd11 phenotypes require NahG-degradable compounds not synthesized via isochorismate.

MeSH Terms
Apoptosis/physiology Apoptosis Regulatory Proteins Arabidopsis/cytology,genetics,metabolism,physiology Arabidopsis Proteins/genetics,physiology Biomarkers Epistasis, Genetic Gene Expression Regulation, Plant Intramolecular Transferases/genetics,physiology Membrane Transport Proteins/genetics,physiology Mixed Function Oxygenases/genetics,physiology Mutation Phenotype Salicylic Acid/metabolism
Chemicals
ACD11 protein, Arabidopsis Apoptosis Regulatory Proteins Arabidopsis Proteins Biomarkers EDS5 protein, Arabidopsis Membrane Transport Proteins Mixed Function Oxygenases salicylate 1-monooxygenase Intramolecular Transferases isochorismate synthase Salicylic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brodersen Peter
Institute of Molecular Biology, Copenhagen University, DK-1353 Copenhagen, Denmark.
Malinovsky Frederikke Gro
Hématy Kian
Newman Mari-Anne
Mundy John
References (47)
47 references, click to expand
  1. Suppression and Restoration of Lesion Formation in Arabidopsis lsd Mutants.
    Plant Cell. 1995 Dec;7(12):2013-2022 PMID: 12242366
  2. Dissection of Arabidopsis Bax inhibitor-1 suppressing Bax-, hydrogen peroxide-, and salicylic acid-induced cell death.
    Plant Cell. 2004 Jan;16(1):21-32 PMID: 14671021
  3. Signals involved in Arabidopsis resistance to Trichoplusia ni caterpillars induced by virulent and avirulent strains of the phytopathogen Pseudomonas syringae.
    Plant Physiol. 2002 Jun;129(2):551-64 PMID: 12068100
  4. The gain-of-function Arabidopsis acd6 mutant reveals novel regulation and function of the salicylic acid signaling pathway in controlling cell death, defenses, and cell growth.
    Plant Cell. 1999 Sep;11(9):1695-708 PMID: 10488236
  5. Production of Salicylic Acid Precursors Is a Major Function of Phenylalanine Ammonia-Lyase in the Resistance of Arabidopsis to Peronospora parasitica.
    Plant Cell. 1996 Feb;8(2):203-212 PMID: 12239383
  6. Salicylic acid induction-deficient mutants of Arabidopsis express PR-2 and PR-5 and accumulate high levels of camalexin after pathogen inoculation.
    Plant Cell. 1999 Aug;11(8):1393-404 PMID: 10449575
  7. Apoptosis/differentiation-inducing effects of vitamin K2 on HL-60 cells: dichotomous nature of vitamin K2 in leukemia cells.
    Leukemia. 2001 Jul;15(7):1111-7 PMID: 11455981
  8. Use of Arabidopsis for genetic dissection of plant defense responses.
    Annu Rev Genet. 1997;31:547-69 PMID: 9442907
  9. Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to several bacterial pathogens and alterations in PR-1 gene expression.
    Plant Cell. 1997 Mar;9(3):305-16 PMID: 9090877
  10. Fumonisin B1-induced cell death in arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways.
    Plant Cell. 2000 Oct;12(10):1823-36 PMID: 11041879
  11. Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase.
    Plant J. 2001 Jan;25(1):67-77 PMID: 11169183
  12. Compromising early salicylic acid accumulation delays the hypersensitive response and increases viral dispersal during lesion establishment in TMV-infected tobacco.
    Plant J. 1997 Nov;12(5):1113-26 PMID: 9418052
  13. Salicylic acid potentiates an agonist-dependent gain control that amplifies pathogen signals in the activation of defense mechanisms.
    Plant Cell. 1997 Feb;9(2):261-70 PMID: 9061956
  14. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
    Methods. 2001 Dec;25(4):402-8 PMID: 11846609
  15. Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein.
    Proc Natl Acad Sci U S A. 1997 May 27;94(11):5961-6 PMID: 9159183
  16. Arabidopsis mutants simulating disease resistance response.
    Cell. 1994 May 20;77(4):565-77 PMID: 8187176
  17. Three unique mutants of Arabidopsis identify eds loci required for limiting growth of a biotrophic fungal pathogen.
    Plant J. 2000 Oct;24(2):205-18 PMID: 11069695
  18. Salicylic Acid: a likely endogenous signal in the resistance response of tobacco to viral infection.
    Science. 1990 Nov 16;250(4983):1002-4 PMID: 17746925
  19. Increase in salicylic Acid at the onset of systemic acquired resistance in cucumber.
    Science. 1990 Nov 16;250(4983):1004-6 PMID: 17746926
  20. Regulation of anthraquinone biosynthesis in cell cultures of Morinda citrifolia.
    J Plant Physiol. 2003 Jun;160(6):607-14 PMID: 12872482
  21. Recent breakthroughs in the study of salicylic acid biosynthesis.
    Trends Plant Sci. 2002 Aug;7(8):332-4 PMID: 12167322
  22. The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance.
    Plant Cell. 1997 Sep;9(9):1573-84 PMID: 9338960
  23. Induction of hydroxycinnamoyl-tyramine conjugates in pepper by Xanthomonas campestris, a plant defense response activated by hrp gene-dependent and hrp gene-independent mechanisms.
    Mol Plant Microbe Interact. 2001 Jun;14(6):785-92 PMID: 11386374
  24. Isochorismate synthase is required to synthesize salicylic acid for plant defence.
    Nature. 2001 Nov 29;414(6863):562-5 PMID: 11734859
  25. Loss of non-host resistance of Arabidopsis NahG to Pseudomonas syringae pv. phaseolicola is due to degradation products of salicylic acid.
    Plant J. 2003 Feb;33(4):733-42 PMID: 12609045
  26. Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway.
    Plant J. 1996 Jul;10(1):71-82 PMID: 8758979
  27. Correlation of defense gene induction defects with powdery mildew susceptibility in Arabidopsis enhanced disease susceptibility mutants.
    Plant J. 1998 Nov;16(4):473-85 PMID: 9881167
  28. Intermediates of salicylic acid biosynthesis in tobacco
    Plant Physiol. 1998 Oct;118(2):565-72 PMID: 9765542
  29. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.
    Genes Dev. 1998 Dec 1;12(23):3703-14 PMID: 9851977
  30. Requirement of salicylic Acid for the induction of systemic acquired resistance.
    Science. 1993 Aug 6;261(5122):754-6 PMID: 17757215
  31. Role of salicylic acid and NIM1/NPR1 in race-specific resistance in arabidopsis.
    Genetics. 2002 Jun;161(2):803-11 PMID: 12072475
  32. Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance.
    Plant Cell. 1991 Oct;3(10):1085-1094 PMID: 12324583
  33. Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco.
    Virology. 1979 Dec;99(2):410-2 PMID: 18631626
  34. Acquired resistance in Arabidopsis.
    Plant Cell. 1992 Jun;4(6):645-56 PMID: 1392589
  35. Genetic evidence that expression of NahG modifies defence pathways independent of salicylic acid biosynthesis in the Arabidopsis-Pseudomonas syringae pv. tomato interaction.
    Plant J. 2003 Nov;36(3):342-52 PMID: 14617091
  36. Overproduction of salicylic acid in plants by bacterial transgenes enhances pathogen resistance.
    Nat Biotechnol. 2000 Jul;18(7):779-83 PMID: 10888849
  37. Rhein induces apoptosis in HL-60 cells via reactive oxygen species-independent mitochondrial death pathway.
    Arch Biochem Biophys. 2003 Oct 15;418(2):99-107 PMID: 14522581
  38. EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family.
    Plant Cell. 2002 Jan;14(1):275-86 PMID: 11826312
  39. Enhanced transcription of the Arabidopsis disease resistance genes RPW8.1 and RPW8.2 via a salicylic acid-dependent amplification circuit is required for hypersensitive cell death.
    Plant Cell. 2003 Jan;15(1):33-45 PMID: 12509520
  40. Recognition and response in the plant immune system.
    Annu Rev Genet. 2003;37:579-609 PMID: 14616074
  41. Cytochrome c release and caspase activation during menadione-induced apoptosis in plants.
    FEBS Lett. 1999 Dec 3;462(3):317-21 PMID: 10622718
  42. The Arabidopsis ssi1 mutation restores pathogenesis-related gene expression in npr1 plants and renders defensin gene expression salicylic acid dependent.
    Plant Cell. 1999 Feb;11(2):191-206 PMID: 9927638
  43. Knockout of Arabidopsis accelerated-cell-death11 encoding a sphingosine transfer protein causes activation of programmed cell death and defense.
    Genes Dev. 2002 Feb 15;16(4):490-502 PMID: 11850411
  44. A central role of salicylic Acid in plant disease resistance.
    Science. 1994 Nov 18;266(5188):1247-50 PMID: 17810266
  45. SALICYLATE HYDROXYLASE, A MONOOXYGENASE REQUIRING FLAVIN ADENINE DINUCLEOTIDE. I. PURIFICATION AND GENERAL PROPERTIES.
    J Biol Chem. 1965 Aug;240:3408-13 PMID: 14321380
  46. Signaling pathway for aloe-emodin-induced apoptosis in human H460 lung nonsmall carcinoma cell.
    Int J Cancer. 2003 Aug 10;106(1):26-33 PMID: 12794753
  47. Pathway of Salicylic Acid Biosynthesis in Healthy and Virus-Inoculated Tobacco.
    Plant Physiol. 1993 Oct;103(2):315-321 PMID: 12231938
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.105.059303
Published
2005-06-00
Epub
2005-00-27
Pages
1037-45
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1150418
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.105.059303 PMC Full Text View journal details
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