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PMID: 15928205 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements.

The Journal of cell biology ·Vol. 169 ·No. 5 ·2005-06-06 ·Pages 777-87

Lin F, Sepich DS, Chen S, Topczewski J, Yin C, Solnica-Krezel L, Hamm H

Abstract

Galpha(12/13) have been implicated in numerous cellular processes, however, their roles in vertebrate gastrulation are largely unknown. Here, we show that during zebrafish gastrulation, suppression of both Galpha(12) and Galpha(13) signaling by overexpressing dominant negative proteins and application of antisense morpholino-modified oligonucleotide translation interference disrupted convergence and extension without changing embryonic patterning. Analyses of mesodermal cell behaviors revealed that Galpha(12/13) are required for cell elongation and efficient dorsalward migration during convergence independent of noncanonical Wnt signaling. Furthermore, Galpha(12/13) function cell-autonomously to mediate mediolateral cell elongation underlying intercalation during notochord extension, likely acting in parallel to noncanonical Wnt signaling. These findings provide the first evidence that Galpha(12) and Galpha(13) have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation.

MeSH Terms
Animals Body Patterning/physiology Cell Communication/physiology Cell Line Cell Movement/physiology Cell Shape/physiology Embryonic Development/physiology GTP-Binding Protein alpha Subunits, G12-G13/genetics,metabolism Gastrula/cytology,metabolism Gene Expression Regulation, Developmental/physiology Humans Intercellular Signaling Peptides and Proteins/metabolism Mesoderm/cytology,metabolism Molecular Sequence Data Receptors, G-Protein-Coupled/genetics,metabolism Signal Transduction/physiology Wnt Proteins Zebrafish/embryology,metabolism
Chemicals
Intercellular Signaling Peptides and Proteins Receptors, G-Protein-Coupled Wnt Proteins GTP-Binding Protein alpha Subunits, G12-G13
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lin Fang
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Sepich Diane S
Chen Songhai
Topczewski Jacek
Yin Chunyue
Solnica-Krezel Lilianna
Hamm Heidi
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200501104
Published
2005-06-06
Epub
2005-00-31
Pages
777-87
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171618
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055101 · United States
NEI NIH HHS · EY10291 · United States
NHLBI NIH HHS · T32 HL007751 · United States
NIGMS NIH HHS · GM55101 · United States
NEI NIH HHS · R01 EY010291 · United States
NCRR NIH HHS · 1S10RR015682 · United States
NHLBI NIH HHS · HL60678 · United States
NHLBI NIH HHS · T32HL07751 · United States
NHLBI NIH HHS · P01 HL060678 · United States
Databases
GENBANK
AY386359, AY386360, AY386361
External Links
PubMed source DOI: 10.1083/jcb.200501104 PMC Full Text View journal details
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