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PMID: 15937219 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila.

Genes & development ·Vol. 19 ·No. 11 ·2005-06-01 ·Pages 1306-14

Blanchette M, Green RE, Brenner SE, Rio DC

Abstract

To gain insight into splicing regulation, we developed a microarray to assay all annotated alternative splicing events in Drosophila melanogaster and identified the alternative splice events controlled by four splicing regulators: dASF/SF2, B52/SRp55, hrp48, and PSI. The number of events controlled by each of these factors was found to be highly variable: dASF/SF2 strongly affects >300 splicing events, whereas PSI strongly affects only 43 events. Pairwise analysis also revealed many instances of splice site usage affected by multiple factors and provides the framework to understand the network controlling the alternatively spliced mRNA isoforms that compose the Drosophila transcriptome.

MeSH Terms
Animals Binding Sites Drosophila melanogaster/genetics RNA Precursors/genetics,metabolism RNA Splicing RNA, Messenger/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction
Chemicals
RNA Precursors RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Blanchette Marco
Department of Molecular and Cell Biology, Center for Integrative Genomics, Plant and Microbial Biology, University of California, Berkeley, 94720-3204 USA.
Green Richard E
Brenner Steven E
Rio Donald C
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1314205
Published
2005-06-01
Pages
1306-14
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1142554
Subset
IM
Grants
NIGMS NIH HHS · R01 GM061987 · United States
NIGMS NIH HHS · R01GM61987 · United States
External Links
PubMed source DOI: 10.1101/gad.1314205 PMC Full Text View journal details
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