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PMID: 1594599 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The rad3+ gene of Schizosaccharomyces pombe is involved in multiple checkpoint functions and in DNA repair.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 11 ·1992-06-01 ·Pages 4952-6

Jimenez G, Yucel J, Rowley R, Subramani S

Abstract

A number of important molecular checkpoints are believed to control the orderly progression of cell cycle events. We have found that the radiation-sensitive Schizosaccharomyces pombe mutant rad3-136 is deficient in two molecular checkpoint functions. Unlike wild-type cells, the mutant cells are unable to arrest in the G2 phase of the cell cycle after DNA damage by gamma-irradiation and are also incapable of maintaining the dependence of mitosis upon the completion of DNA synthesis. An S. pombe genomic clone that complements the UV sensitivity of the rad3-136 mutant completely restores the missing checkpoint functions. The rad3+ gene is also likely to play a role in DNA repair.

Related Genes
rad3
MeSH Terms
Benomyl/pharmacology Cell Cycle Cloning, Molecular DNA Ligases/metabolism DNA Repair DNA Replication Gamma Rays Genes, Fungal Genetic Complementation Test Hydroxyurea/pharmacology Mitosis Schizosaccharomyces/cytology,genetics
Chemicals
DNA Ligases Benomyl Hydroxyurea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jimenez G
Department of Biology, University of California, San Diego, La Jolla 92093.
Yucel J
Rowley R
Subramani S
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.11.4952
Published
1992-06-01
Pages
4952-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49206
Subset
IM
Grants
NCI NIH HHS · CA40245 · United States
NIGMS NIH HHS · GM31253 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.11.4952 PMC Full Text View journal details
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