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PMID: 15951838 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

FGF-2 controls the differentiation of resident cardiac precursors into functional cardiomyocytes.

The Journal of clinical investigation ·Vol. 115 ·No. 7 ·2005-07-00 ·Pages 1724-33

Rosenblatt-Velin N, Lepore MG, Cartoni C, Beermann F, Pedrazzini T

Abstract

Recent evidence suggests that the heart possesses a greater regeneration capacity than previously thought. In the present study, we isolated undifferentiated precursors from the cardiac nonmyocyte cell population of neonatal hearts, expanded them in culture, and induced them to differentiate into functional cardiomyocytes. These cardiac precursors appear to express stem cell antigen-1 and demonstrate characteristics of multipotent precursors of mesodermal origin. Following infusion into normal recipients, these cells home to the heart and participate in physiological and pathophysiological cardiac remodeling. Cardiogenic differentiation in vitro and in vivo depends on FGF-2. Interestingly, this factor does not control the number of precursors but regulates the differentiation process. These findings suggest that, besides its angiogenic actions, FGF-2 could be used in vivo to facilitate the mobilization and differentiation of resident cardiac precursors in the treatment of cardiac diseases.

MeSH Terms
Animals Antigens, Ly/metabolism Cell Differentiation/physiology Cells, Cultured Fibroblast Growth Factor 2/deficiency,genetics,physiology Membrane Proteins/metabolism Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Multipotent Stem Cells/cytology,physiology Myoblasts, Cardiac/cytology,physiology Myocytes, Cardiac/cytology,physiology Stem Cell Transplantation
Chemicals
Antigens, Ly Ly6a protein, mouse Membrane Proteins Fibroblast Growth Factor 2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rosenblatt-Velin Nathalie
Department of Medicine, University of Lausanne Medical School, Lausanne, Switzerland. Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland.
Lepore Mario G
Cartoni Cristina
Beermann Friedrich
Pedrazzini Thierry
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI23418
Published
2005-07-00
Epub
2005-00-09
Pages
1724-33
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC1143587
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI23418 PMC Full Text View journal details
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