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PMID: 15957214 Published · ppublish English Journal Article

Molecular cloning of the c locus of Zea mays: a locus regulating the anthocyanin pathway.

The EMBO journal ·Vol. 5 ·No. 5 ·1986-05-00 ·Pages 829-33

Paz-Ares J, Wienand U, Peterson PA, Saedler H

Abstract

The c locus of Zea mays, involved in the regulation of anthocyanin biosynthesis, has been cloned by transposon tagging. A clone (# 18En) containing a full size En1 element was initially isolated from the En element-induced mutable allele c-m668655. Sequences of clone # 18En flanking the En1 element were used to clone other c mutants, whose structure was predicted genetically. Clone #23En (isolated from c-m668613) contained a full size En1 element, clone #3Ds (isolated from c-m2) a Ds element and clone # 5 (isolated from c+) had no element on the cloned fragment. From these data we conclude that the clones obtained contain at least part of the c locus. Preliminary data on transcript analysis using a 1-kb DNA fragment from wild-type clone # 5 showed that at least three transcripts are encoded by that part of the locus, indicating that c is a complex locus.

MeSH Terms
Alleles Anthocyanins/biosynthesis,genetics Blotting, Southern Cloning, Molecular DNA Transposable Elements/genetics Genes, Plant/genetics Zea mays/genetics
Chemicals
Anthocyanins DNA Transposable Elements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Paz-Ares J
Max-Planck Institut für Züchtungsforschung, Egelspfad, D-5000 Köln 30, FRG.
Wienand U
Peterson P A
Saedler H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-05-00
Pages
829-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166870
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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