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PMID: 15987933 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

NMDA receptor subunit-dependent [Ca2+] signaling in individual hippocampal dendritic spines.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 25 ·No. 26 ·2005-06-29 ·Pages 6037-46

Sobczyk A, Scheuss V, Svoboda K

Abstract

Ca2+ influx through synaptic NMDA receptors (NMDA-Rs) triggers a variety of adaptive cellular processes. To probe NMDA-R-mediated [Ca2+] signaling, we used two-photon glutamate uncaging to stimulate NMDA-Rs on individual dendritic spines of CA1 pyramidal neurons in rat brain slices. We measured NMDA-R currents at the soma and NMDA-R-mediated [Ca2+] transients in stimulated spines (Delta[Ca2+]). Uncaging-evoked NMDA-R current amplitudes were independent of the size of the stimulated spine, implying that smaller spines contain higher densities of functional NMDA-Rs. The ratio of Delta[Ca2+] over NMDA-R current was highly variable (factor of 10) across spines, especially for small spines. These differences were not explained by heterogeneity in spine sizes or diffusional coupling between spines and their parent dendrites. In addition, we find that small spines have NMDA-R currents that are sensitive to NMDA-R NR2B subunit-specific antagonists. With block of NR2B-containing receptors, the range of Delta[Ca2+]/NMDA-R current ratios and their average value were much reduced. Our data suggest that individual spines can regulate the subunit composition of their NMDA-Rs and the effective fractional Ca2+ current through these receptors.

MeSH Terms
Animals Calcium Signaling/drug effects,physiology Dendritic Spines/drug effects,physiology Evoked Potentials Excitatory Amino Acid Antagonists/pharmacology Hippocampus/physiology Quinoxalines/pharmacology Receptors, N-Methyl-D-Aspartate/physiology Synapses/drug effects,physiology
Chemicals
Excitatory Amino Acid Antagonists NR2B NMDA receptor Quinoxalines Receptors, N-Methyl-D-Aspartate 2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sobczyk Aleksander
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Scheuss Volker
Svoboda Karel
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.1221-05.2005
Published
2005-06-29
Pages
6037-46
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6725044
Subset
IM
External Links
PubMed source DOI: 10.1523/JNEUROSCI.1221-05.2005 PMC Full Text View journal details
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