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PMID: 15993643 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

A versatile vector system for multiple gene expression in plants.

Trends in plant science ·Vol. 10 ·No. 8 ·2005-08-00 ·Pages 357-61

Chung SM, Frankman EL, Tzfira T

Abstract

Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.

MeSH Terms
Cloning, Molecular/methods Gene Expression Regulation, Plant Multigene Family Plants/genetics Promoter Regions, Genetic Terminator Regions, Genetic/genetics
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chung Sang-Min
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, 11794, USA.
Frankman Ellen L
Tzfira Tzvi
Article Info
Journal
Trends in plant science
Abbr.
Trends Plant Sci
ISSN
1360-1385
Published
2005-08-00
Pages
357-61
Language
English
Region
England
NLM ID
9890299
Subset
IM
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