Home LiteratureArticle Details
PMID: 1599394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

L-arginine transport is increased in macrophages generating nitric oxide.

The Biochemical journal ·Vol. 284 ( Pt 1) ·1992-05-15 ·Pages 15-8

Bogle RG, Baydoun AR, Pearson JD, Moncada S, Mann GE

Abstract

Transport of L-arginine and nitrite production were examined in the murine macrophage cell line J774. Bacterial lipopolysaccharide (LPS) induced a dose- and time-dependent stimulation of nitrite production, which was further increased in the presence of interferon-gamma. Nitrite synthesis was absolutely dependent on extracellular L-arginine and inhibited in the presence of L-lysine or L-ornithine. In unactivated J774 cells L-arginine transport was saturable, with an apparent Km of 0.14 +/- 0.04 mM and Vmax. of 15 +/- 2 nmol/h per 10(6) cells. LPS (1 microgram/ml) induced a time-dependent stimulation of L-arginine transport, and after 24 h the Vmax. increased to 34 +/- 2 nmol/h per 10(6) cells. These findings indicate that activation of J774 cells with LPS produces an increase in both L-arginine transport and nitrite synthesis. The elevated rate of L-arginine transport in activated J774 cells may provide a mechanism for sustained substrate supply during enhanced utilization of L-arginine for the generation of NO.

MeSH Terms
Animals Arginine/pharmacokinetics Biological Transport Cells, Cultured Extracellular Space/metabolism Interferon-gamma/pharmacology Kinetics Lipopolysaccharides Macrophages/metabolism Mice Nitric Oxide/metabolism Nitrites/metabolism Recombinant Proteins
Chemicals
Lipopolysaccharides Nitrites Recombinant Proteins Nitric Oxide Interferon-gamma Arginine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bogle R G
Vascular Biology Research Centre, Biomedical Sciences Division, King's College, London, U.K.
Baydoun A R
Pearson J D
Moncada S
Mann G E
References (31)
31 references, click to expand
  1. Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein.
    J Biol Chem. 1991 Dec 5;266(34):22789-91 PMID: 1720773
  2. Enhancement of glutathione levels in mouse peritoneal macrophages by sodium arsenite, cadmium chloride and glucose/glucose oxidase.
    Biochim Biophys Acta. 1991 Apr 17;1092(2):175-9 PMID: 2018783
  3. Glucocorticoids inhibit the induction of nitric oxide synthase in macrophages.
    Biochem Biophys Res Commun. 1990 Nov 15;172(3):1246-52 PMID: 1700905
  4. L-arginine-dependent production of nitrogen oxides by rat pulmonary macrophages.
    Eur J Pharmacol. 1991 Aug 6;200(2-3):205-9 PMID: 1782986
  5. Induction of cationic amino acid transport activity in mouse peritoneal macrophages by lipopolysaccharide.
    Biochim Biophys Acta. 1991 Oct 14;1069(1):46-52 PMID: 1932048
  6. Induction of cystine transport activity in mouse peritoneal macrophages.
    J Exp Med. 1987 Mar 1;165(3):628-40 PMID: 2880923
  7. L-arginine is required for expression of the activated macrophage effector mechanism causing selective metabolic inhibition in target cells.
    J Immunol. 1987 Jan 15;138(2):550-65 PMID: 2432129
  8. The transport of cationic amino acids across the plasma membrane of mammalian cells.
    Biochim Biophys Acta. 1985 Dec 9;822(3-4):355-74 PMID: 2933076
  9. Synthesis of nitrite and nitrate in murine macrophage cell lines.
    Cancer Res. 1987 Nov 1;47(21):5590-4 PMID: 3117354
  10. Regulation of macrophage functions by L-arginine.
    J Exp Med. 1989 Mar 1;169(3):1021-9 PMID: 2538541
  11. Changes in neutral amino acid transport activity in myeloid leukemia cells differentiated by lipopolysaccharide.
    Biochim Biophys Acta. 1989 Aug 7;983(2):259-63 PMID: 2503038
  12. Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells.
    J Exp Med. 1989 May 1;169(5):1543-55 PMID: 2497225
  13. Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate.
    Biochemistry. 1988 Nov 29;27(24):8706-11 PMID: 3242600
  14. Nitric oxide: a cytotoxic activated macrophage effector molecule.
    Biochem Biophys Res Commun. 1988 Nov 30;157(1):87-94 PMID: 3196352
  15. Arginine metabolism in wounds.
    Am J Physiol. 1988 Apr;254(4 Pt 1):E459-67 PMID: 3354662
  16. The cell biology of macrophage activation.
    Annu Rev Immunol. 1984;2:283-318 PMID: 6100475
  17. Secretory products of macrophages.
    J Clin Invest. 1987 Feb;79(2):319-26 PMID: 3543052
  18. Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7738-42 PMID: 3906650
  19. Induction and activity of NO synthase in bone-marrow-derived macrophages are independent of Ca2+.
    Biochem J. 1990 Sep 1;270(2):351-6 PMID: 1698056
  20. Metabolic fate of L-arginine in relation to microbiostatic capability of murine macrophages.
    J Clin Invest. 1990 Jan;85(1):264-73 PMID: 2404026
  21. L-arginine-dependent reactive nitrogen intermediates as mediators of tumor cell killing by activated macrophages.
    Cancer Res. 1990 Mar 1;50(5):1421-5 PMID: 2302707
  22. Glutathione metabolism in resting and phagocytizing peritoneal macrophages.
    J Biol Chem. 1982 Feb 25;257(4):2002-8 PMID: 6120172
  23. Enhancement of carrier-mediated transport after immunologic activation of peritoneal macrophages.
    J Immunol. 1977 May;118(5):1827-35 PMID: 404359
  24. Microenvironmental arginine depletion by macrophages in vivo.
    Br J Cancer. 1979 Jun;39(6):613-20 PMID: 444403
  25. Activated macrophages kill tumour cells by releasing arginase.
    Nature. 1978 Jun 29;273(5665):758-9 PMID: 661984
  26. Membrane transport by macrophages in suspension and adherent to glass.
    J Cell Physiol. 1977 Aug;92(2):249-55 PMID: 881435
  27. The gamma-glutamyl cycle: a possible transport system for amino acids.
    Proc Natl Acad Sci U S A. 1970 Nov;67(3):1248-55 PMID: 5274454
  28. Effects of serum on membrane transport. I. Separation and preliminary characterization of factors which depress lysine or stimulate adenosine transport in rabbit alveolar macrophages.
    J Exp Med. 1973 Feb 1;137(2):359-68 PMID: 4119589
  29. Adaptive regulation of amino acid transport across the cell membrane in avian and mammalian tissues.
    Biochim Biophys Acta. 1975 Oct 6;406(2):264-79 PMID: 1238115
  30. Membrane transport in the rabbit alveolar macrophage. The specificity and characteristics of amino acid transport systems.
    Biochim Biophys Acta. 1971 Jul 6;241(1):155-69 PMID: 5125243
  31. Purification of nitric oxide synthase from rat macrophages.
    J Biol Chem. 1991 Jul 5;266(19):12544-7 PMID: 1712021
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2840015
Published
1992-05-15
Pages
15-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132690
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2840015 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2