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PMID: 15998801 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

A microtubule-dependent zone of active RhoA during cleavage plane specification.

The Journal of cell biology ·Vol. 170 ·No. 1 ·2005-07-04 ·Pages 91-101

Bement WM, Benink HA, von Dassow G

Abstract

Cytokinesis in animal cells results from the assembly and constriction of a circumferential array of actin filaments and myosin-2. Microtubules of the mitotic apparatus determine the position at which the cytokinetic actomyosin array forms, but the molecular mechanisms by which they do so remain unknown. The small GTPase RhoA has previously been implicated in cytokinesis. Using four-dimensional microscopy and a probe for active RhoA, we show that active RhoA concentrates in a precisely bounded zone before cytokinesis and is independent of actin assembly. Cytokinetic RhoA activity zones are common to four echinoderm species, the vertebrate Xenopus laevis, and the highly asymmetric cytokinesis accompanying meiosis. Microtubules direct the formation and placement of the RhoA activity zone, and the zone is repositioned after physical spindle displacement. We conclude that microtubules specify the cytokinetic apparatus via a dynamic zone of local RhoA activity.

MeSH Terms
Actin Cytoskeleton/metabolism Animals Cleavage Stage, Ovum/cytology,physiology Cytokinesis/physiology Embryo, Nonmammalian/cytology,metabolism Female Intracellular Signaling Peptides and Proteins/metabolism Meiosis/physiology Microtubules/metabolism Myosins/metabolism Recombinant Fusion Proteins/genetics,metabolism Sea Urchins Species Specificity Spindle Apparatus/metabolism Starfish Strongylocentrotus purpuratus Xenopus laevis rhoA GTP-Binding Protein/metabolism
Chemicals
Intracellular Signaling Peptides and Proteins Recombinant Fusion Proteins Myosins rhoA GTP-Binding Protein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bement William M
Center for Cell Dynamics, Friday Harbor Laboratories, University of Washington, Friday Harbor, WA 98250, USA. [email protected]
Benink Hélène A
von Dassow George
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200501131
Email
[email protected]
Published
2005-07-04
Pages
91-101
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171391
Subset
IM
Grants
NIGMS NIH HHS · P50 GM066050 · United States
NIGMS NIH HHS · R01 GM052932 · United States
NIGMS NIH HHS · GM52932 · United States
NIGMS NIH HHS · GM66050 · United States
External Links
PubMed source DOI: 10.1083/jcb.200501131 PMC Full Text View journal details
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