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PMID: 16000162 Published · ppublish English Journal Article

RanBPM is an L1-interacting protein that regulates L1-mediated mitogen-activated protein kinase activation.

Journal of neurochemistry ·Vol. 94 ·No. 4 ·2005-08-00 ·Pages 1102-10

Cheng L, Lemmon S, Lemmon V

Abstract

A yeast two-hybrid screen using the last 28 amino acids of the cytoplasmic domain of the neural cell adhesion molecule L1 identified RanBPM as an L1-interacting protein. RanBPM associates with L1 in vivo and the N-terminal region of RanBPM (N-RanBPM), containing the SPRY domain, is sufficient for the interaction with L1 in a glutathione S-transferase pull-down assay. L1 antibody patching dramatically changes the subcellular localization of N-RanBPM in transfected COS cells. Overexpression of N-RanBPM in COS cells reduces L1-triggered extracellular signal-regulated kinase 1/2 activation by 50% and overexpression of N-RanBPM in primary neurons inhibits L1-mediated neurite outgrowth and branching. These data suggest that RanBPM is an adaptor protein that links L1 to the extracellular signal-regulated kinase/MAPK pathway.

MeSH Terms
Adaptor Proteins, Signal Transducing Animals COS Cells Chlorocebus aethiops Cytoskeletal Proteins Drug Interactions Enzyme Activation/physiology Leukocyte L1 Antigen Complex/physiology Mitogen-Activated Protein Kinases/metabolism Neurites/physiology Nuclear Proteins/genetics,physiology Protein Structure, Tertiary/physiology Tissue Distribution Two-Hybrid System Techniques ran GTP-Binding Protein/genetics,physiology
Chemicals
Adaptor Proteins, Signal Transducing Cytoskeletal Proteins Leukocyte L1 Antigen Complex Nuclear Proteins Ran binding protein 9 Mitogen-Activated Protein Kinases ran GTP-Binding Protein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cheng Ling
Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio, USA.
Lemmon Sandra
Lemmon Vance
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Article Info
Journal
Journal of neurochemistry
Abbr.
J Neurochem
ISSN
0022-3042
DOI
10.1111/j.1471-4159.2005.03254.x
Published
2005-08-00
Epub
2005-00-05
Pages
1102-10
Language
English
Region
England
NLM ID
2985190R
PMCID
PMC2424128
Subset
IM
Grants
NEI NIH HHS · R01 EY005285 · United States
NEI NIH HHS · R01 EY005285-23 · United States
NICHD NIH HHS · R01 HD039884 · United States
NICHD NIH HHS · R01 HD039884-06 · United States
External Links
PubMed source DOI: 10.1111/j.1471-4159.2005.03254.x PMC Full Text View journal details
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