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PMID: 16025326 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characteristics of NADPH oxidase genes (Nox2, p22, p47, and p67) and Nox4 gene expressed in blood cells of juvenile Ciona intestinalis.

Immunogenetics ·Vol. 57 ·No. 7 ·2005-08-00 ·Pages 520-34

Inoue Y, Ogasawara M, Moroi T, Satake M, Azumi K, Moritomo T, Nakanishi T

Abstract

To illuminate the origins of NADPH oxidase (Nox), we identified cDNA clones encoding Nox2, Nox4, p22 phagocyte oxidase (phox), p47phox, and p67phox in a chordate phylogenetically distant to the vertebrates, the sea squirt Ciona intestinalis. We also examined the spatiotemporal expression of these genes in embryos and juveniles. The sequences of the Nox2, Nox4, p22phox, p47phox, and p67phox cDNAs contained open reading frames encoding 581, 811, 175, 461, and 515 amino acids, respectively. The level of identities between the deduced Nox2, Nox4, p22phox, p47phox, and p67phox amino acid sequences and their corresponding human components were 54.0, 31.0, 44.4, 36.0, and 26.2%, respectively. Despite these low identities, the functional domains of the C. intestinalis and human NADPH oxidase and Nox4 are highly conserved. The genomic organizations of the components of the NADPH oxidase gene except for p67phox (a single exon gene) and the Nox4 gene in C. intestinalis are highly similar to those of the corresponding human NADPH oxidase genes. Further, the analyzed part of the C. intestinalis genome and EST database do not seem to present p40phox and Nox5. The Nox2, p22phox, p47phox, and p67phox genes were specifically expressed in the blood cells of juveniles. The Nox4 gene was expressed in blood cells and endostyle of juveniles. These results suggest that C. intestinalis NADPH oxidase components possess potential functional activities similar to those of human, but the manner in which cytosolic phox proteins in C. intestinalis interact is different from that in human.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Ciona intestinalis/enzymology,genetics,growth & development DNA, Complementary/genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Genome Humans In Situ Hybridization Models, Biological Molecular Sequence Data NADPH Oxidase 4 NADPH Oxidases/blood,chemistry,genetics Phylogeny Sequence Homology, Amino Acid Species Specificity
Chemicals
DNA, Complementary NADPH Oxidase 4 NADPH Oxidases NOX4 protein, human
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Inoue Yuuki
Laboratory of Fish Pathology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-8510, Japan, [email protected]
Ogasawara Michio
Moroi Takuma
Satake Masanobu
Azumi Kaoru
Moritomo Tadaaki
Nakanishi Teruyuki
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/s00251-005-0010-4
Email
[email protected]
Published
2005-08-00
Epub
2005-00-29
Pages
520-34
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/s00251-005-0010-4 View journal details
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