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PMID: 16028888 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

Surfaces and orientations: much to FRET about?

Accounts of chemical research ·Vol. 38 ·No. 7 ·2005-07-00 ·Pages 542-8

Rasnik I, McKinney SA, Ha T

Abstract

Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this Account, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.

MeSH Terms

Biotin Fluorescence Resonance Energy Transfer/methods Molecular Conformation Protein Binding Quartz Serum Albumin, Bovine Streptavidin

Chemicals

Quartz Serum Albumin, Bovine Biotin Streptavidin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rasnik Ivan

Physics Department and Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA.

McKinney Sean A

Ha Taekjip

Article Info

Journal

Accounts of chemical research

Abbr.

Acc Chem Res

ISSN

0001-4842

DOI

10.1021/ar040138c

Published

2005-07-00

Pages

542-8

Language

English

Region

United States

NLM ID

0157313

Subset

Grants

NIGMS NIH HHS · R01 GM065367 · United States

NIGMS NIH HHS · GM065367 · United States

NIGMS NIH HHS · T32GM008276 · United States

External Links

PubMed source DOI: 10.1021/ar040138c View journal details

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Surfaces and orientations: much to FRET about?

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