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PMID: 160408 Published · ppublish English Journal Article

Chemiosmotic coupling in Methanobacterium thermoautotrophicum: hydrogen-dependent adenosine 5'-triphosphate synthesis by subcellular particles.

Journal of bacteriology ·Vol. 140 ·No. 3 ·1979-12-00 ·Pages 1081-9

Doddema HJ, van der Drift C, Vogels GD, Veenhuis M

Abstract

Hydrogenase and the adenosine 5'-triphosphate (ATP) synthetase complex, two enzymes essential in ATP generation in Methanobacterium thermoautotrophicum, were localized in internal membrane systems as shown by cytochemical techniques. Membrane vesicles from this organism possessed hydrogenase and adenosine triphosphatase (ATPase) activity and synthesized ATP driven by hydrogen oxidation or a potassium gradient. ATP synthesis depended on anaerobic conditions and could be inhibited in membrane vesicles by uncouplers, nigericin, or the ATPase inhibitor N,N'-dicyclohexylcarbodiimide. The presence of an adenosine 5'-diphosphate-ATP translocase was postulated. With fluorescent dyes, a membrane potential and pH gradient were demonstrated.

MeSH Terms
Adenosine Triphosphatases/metabolism Adenosine Triphosphate/biosynthesis Adenylate Kinase/metabolism Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Dicyclohexylcarbodiimide/pharmacology Dinitrophenols/pharmacology Euryarchaeota/metabolism,ultrastructure Hydrogen/metabolism Intracellular Membranes/metabolism Nigericin/pharmacology Oxidoreductases/metabolism Potassium/metabolism Subcellular Fractions
Chemicals
Dinitrophenols Dicyclohexylcarbodiimide Carbonyl Cyanide m-Chlorophenyl Hydrazone Hydrogen Adenosine Triphosphate Oxidoreductases Adenylate Kinase Adenosine Triphosphatases Nigericin Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Doddema H J
van der Drift C
Vogels G D
Veenhuis M
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.140.3.1081-1089.1979
Published
1979-12-00
Pages
1081-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216755
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.140.3.1081-1089.1979 PMC Full Text View journal details
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