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PMID: 16061697 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The epidermal barrier function is dependent on the serine protease CAP1/Prss8.

The Journal of cell biology ·Vol. 170 ·No. 3 ·2005-08-01 ·Pages 487-96

Leyvraz C, Charles RP, Rubera I, Guitard M, Rotman S, Breiden B, Sandhoff K, Hummler E

Abstract

Serine proteases are proteolytic enzymes that are involved in the regulation of various physiological processes. We generated mice lacking the membrane-anchored channel-activating serine protease (CAP) 1 (also termed protease serine S1 family member 8 [Prss8] and prostasin) in skin, and these mice died within 60 h after birth. They presented a lower body weight and exhibited severe malformation of the stratum corneum (SC). This aberrant skin development was accompanied by an impaired skin barrier function, as evidenced by dehydration and skin permeability assay and transepidermal water loss measurements leading to rapid, fatal dehydration. Analysis of differentiation markers revealed no major alterations in CAP1/Prss8-deficient skin even though the epidermal deficiency of CAP1/Prss8 expression disturbs SC lipid composition, corneocyte morphogenesis, and the processing of profilaggrin. The examination of tight junction proteins revealed an absence of occludin, which did not prevent the diffusion of subcutaneously injected tracer (approximately 600 D) toward the skin surface. This study shows that CAP1/Prss8 expression in the epidermis is crucial for the epidermal permeability barrier and is, thereby, indispensable for postnatal survival.

MeSH Terms
Animals Cell Differentiation/physiology Dehydration/genetics,mortality Epidermis/enzymology,pathology,physiology Filaggrin Proteins Intermediate Filament Proteins/biosynthesis Lipid Metabolism Membrane Proteins/metabolism Mice Mice, Knockout Occludin Permeability Serine Endopeptidases/genetics,physiology Skin Abnormalities/genetics,pathology Tight Junctions/physiology
Chemicals
Filaggrin Proteins Intermediate Filament Proteins Membrane Proteins Occludin Ocln protein, mouse Serine Endopeptidases prostasin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Leyvraz Céline
Département de Pharmacologie et de Toxicologie, Université de Lausanne, CH-1005 Lausanne, Switzerland.
Charles Roch-Philippe
Rubera Isabelle
Guitard Marjorie
Rotman Samuel
Breiden Bernadette
Sandhoff Konrad
Hummler Edith
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200501038
Published
2005-08-01
Pages
487-96
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171460
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200501038 PMC Full Text View journal details
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