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PMID: 16077006 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Variant histone H3.3 is deposited at sites of nucleosomal displacement throughout transcribed genes while active histone modifications show a promoter-proximal bias.

Genes & development ·Vol. 19 ·No. 15 ·2005-08-01 ·Pages 1761-6

Wirbelauer C, Bell O, Schübeler D

Abstract

Deposition of variant histones provides a mechanism to reset and to potentially specify chromatin states. We determined the distribution of H3 and its variant H3.3 relative to chromatin structure and elongating polymerase. H3.3 is enriched throughout active genes similar to polymerase, yet its distribution is very distinct from that of several euchromatic histone modifications, which are highly biased toward the 5' part of active genes. Upon gene induction we observe displacement of both H3 and H3.3 followed by selective deposition of H3.3. These results support a model in which H3.3 deposition compensates for transcription-coupled nucleosomal displacement yet does not predetermine tail modifications.

MeSH Terms
Animals Drosophila Fluorescent Antibody Technique Histones/metabolism Kinetics Nucleosomes/metabolism Promoter Regions, Genetic
Chemicals
Histones Nucleosomes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wirbelauer Christiane
Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
Bell Oliver
Schübeler Dirk
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.347705
Published
2005-08-01
Pages
1761-6
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1182337
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.347705 PMC Full Text View journal details
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