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PMID: 16079231 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

The Ama1-directed anaphase-promoting complex regulates the Smk1 mitogen-activated protein kinase during meiosis in yeast.

Genetics ·Vol. 171 ·No. 3 ·2005-11-00 ·Pages 901-11

McDonald CM, Cooper KF, Winter E

Abstract

Smk1 is a meiosis-specific MAPK homolog in Saccharomyces cerevisiae that regulates the postmeiotic program of spore formation. Similar to other MAPKs, it is activated via phosphorylation of the T-X-Y motif in its regulatory loop, but the signals controlling Smk1 activation have not been defined. Here we show that Ama1, a meiosis-specific activator of the anaphase-promoting complex/cyclosome (APC/C), promotes Smk1 activation during meiosis. A weakened allele of CDC28 suppresses the sporulation defect of an ama1 null strain and increases the activation state of Smk1. The function of Ama1 in regulating Smk1 is independent of the FEAR network, which promotes exit from mitosis and exit from meiosis I through the Cdc14 phosphatase. The data indicate that Cdc28 and Ama1 function in a pathway to trigger Smk1-dependent steps in spore morphogenesis. We propose that this novel mechanism for controlling MAPK activation plays a role in coupling the completion of meiosis II to gamete formation.

MeSH Terms
Anaphase-Promoting Complex-Cyclosome CDC28 Protein Kinase, S cerevisiae/physiology Cdc20 Proteins Cell Cycle Proteins/physiology Gene Expression Regulation, Fungal/physiology Meiosis/physiology Mitogen-Activated Protein Kinases/genetics,metabolism Mutation Protein Tyrosine Phosphatases/physiology Saccharomyces cerevisiae/cytology,enzymology,genetics Saccharomyces cerevisiae Proteins/genetics,metabolism,physiology Signal Transduction/physiology Spores, Fungal/genetics,growth & development Ubiquitin-Protein Ligase Complexes/physiology
Chemicals
Ama1 protein, S cerevisiae CDC14 protein, S cerevisiae Cdc20 Proteins Cell Cycle Proteins Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome CDC28 Protein Kinase, S cerevisiae Mitogen-Activated Protein Kinases Smk1 protein, S cerevisiae Protein Tyrosine Phosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McDonald Christine M
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA.
Cooper Katrina F
Winter Edward
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.105.045567
Published
2005-11-00
Epub
2005-00-03
Pages
901-11
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1456836
Subset
IM
Grants
NIGMS NIH HHS · R01 GM061817 · United States
NIGMS NIH HHS · GM061817 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1534/genetics.105.045567 PMC Full Text View journal details
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