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PMID: 1608402 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides.

Molecular biology reports ·Vol. 16 ·No. 2 ·1992-05-00 ·Pages 91-7

Griffith AJ, Craft J, Evans J, Mimori T, Hardin JA

Abstract

cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.

MeSH Terms
Amino Acid Sequence Antigens, Nuclear Base Sequence Blotting, Southern Cloning, Molecular DNA DNA Helicases DNA-Binding Proteins/genetics Humans Ku Autoantigen Molecular Sequence Data Multigene Family Nuclear Proteins/genetics Polymerase Chain Reaction Restriction Mapping
Chemicals
Antigens, Nuclear DNA-Binding Proteins Nuclear Proteins DNA DNA Helicases XRCC5 protein, human Xrcc6 protein, human Ku Autoantigen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Griffith A J
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
Craft J
Evans J
Mimori T
Hardin J A
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00419754
Published
1992-05-00
Pages
91-7
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
Grants
NIAID NIH HHS · AI26853 · United States
NIAMS NIH HHS · AR32549 · United States
NIAMS NIH HHS · AR40072 · United States
External Links
PubMed source DOI: 10.1007/BF00419754 View journal details
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