Home LiteratureArticle Details
PMID: 16094980 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical mapping of bacteriophage P2 mutations and their relation to the genetic map.

Molecular & general genetics : MGG ·Vol. 136 ·No. 2 ·1975-00-00 ·Pages 139-49

Chattoraj DK, Younghusband HB, Inman RB

Abstract

Three new deletion mutants and an insertion mutant of E. coli bacteriophage P2, del2, vir79, del4 and sig5, were mapped by the electron microscope heteroduplex method. The deletions were found to cover 45.5-51.6%, 75.6-76.7% and 92.3-99.3% respectively of P2 DNA while sig5 represented a 3.7% insertion at 78.6% from the left end. The region covering 75.9-76.7% of P2 DNA is also deleted in the two previously characterized immunity insensitive variants of P2, vir22 and Hy dis. This region may identify the portion of the genome responsible for immunity. The physical and genetic maps of P2 were previously found to be colinear with respect to the two mutations vir22 and vir37. This relationship is confirmed by the position of del2.

MeSH Terms
Bacteriophage P2/genetics DNA, Viral/genetics Genome, Viral Microscopy, Electron, Transmission Mutation Nucleic Acid Denaturation Nucleic Acid Heteroduplexes/genetics Nucleic Acid Hybridization Physical Chromosome Mapping Sequence Deletion
Chemicals
DNA, Viral Nucleic Acid Heteroduplexes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chattoraj D K
Biophysics Laboratory and Biochemistry Department, University of Wisconsin, Madison, Wisconsin 53706, USA.
Younghusband H B
Inman R B
References (16)
16 references, click to expand
  1. Position of two deletion mutations on the physical map of bacteriophage P2.
    J Mol Biol. 1972 May 28;66(3):423-34 PMID: 5037022
  2. A variant of phage P2 originating in Escherichia coli, strain B.
    Virology. 1959 Jan;7(1):112-26 PMID: 13636059
  3. Electron microscope heteroduplex mapping of P2 Hy dis bacteriophage DNA.
    Virology. 1973 Sep;55(1):174-82 PMID: 4580730
  4. On the process of cellular division in Escherichia coli: replication of the bacterial chromosome under control of prophage P2.
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2407-11 PMID: 4944622
  5. LYSOGENIC CONVERSION BY BACTERIOPHAGE P2 RESULTING IN AN INCREASED SENSITIVITY OF ESCHERICHIA COLI TO 5-FLUORODEOXYURIDINE.
    Biochim Biophys Acta. 1964 Aug 12;87:631-40 PMID: 14220693
  6. The electronmicroscopy of DNA.
    Annu Rev Biochem. 1974;43(0):605-19 PMID: 4137265
  7. Calcium sensitive and other mutants of bacteriophage P2.
    J Gen Virol. 1969 Jul;5(1):97-104 PMID: 4898650
  8. On the control of transcription in bacteriophage P2.
    Virology. 1971 Dec;46(3):620-33 PMID: 4944857
  9. Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi.
    J Mol Biol. 1971 Mar 14;56(2):403-23 PMID: 5551396
  10. Excision-deficient mutants of bacteriophage P2.
    Virology. 1972 Jul;49(1):180-7 PMID: 4556923
  11. Tandem duplication in bacteriophage P2: electron microscopic mapping.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):311-4 PMID: 4521804
  12. Genetic map of bacteriophage P2.
    Virology. 1969 Dec;39(4):839-60 PMID: 4902258
  13. Deletions in bacteriophage P2. Circularity of the genetic map and its orientation relative to the DNA denaturation map.
    Mol Gen Genet. 1975;136(2):107-37 PMID: 16094979
  14. Abortive induction of bacteriophage P2.
    Virology. 1968 Sep;36(1):87-103 PMID: 4876153
  15. Constitutive expression of bacteriophage P2 early genes resulting from a tandem duplication.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):315-9 PMID: 4521805
  16. Tandem genetic duplications in a derivative of phage lambda. II. Evidence for structure and location of endpoints.
    J Mol Biol. 1972 Apr 14;65(3):401-12 PMID: 5023665
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00272035
Published
1975-00-00
Pages
139-49
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00272035
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2