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PMID: 16147858 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of Nox genes in rat organs, mouse oocytes, and sea urchin eggs.

DNA sequence : the journal of DNA sequencing and mapping ·Vol. 16 ·No. 2 ·2005-04-00 ·Pages 83-8

Maru Y, Nishino T, Kakinuma K

Abstract

Degenerate primers were designed to isolate new homologs of Nox family genes in rat organs and sea urchin eggs. The primers were capable of amplifying Nox1, Nox2, Nox3, Nox4, Duox1 and Duox2 but not Nox5, and failed to isolate novel homologs in rat. However, a novel homolog (named as Nox-U1) was identified in sea urchin eggs. In the most conserved region (amino acid 336--417 in human Nox2) Nox-U1 has the highest identity with Nox2, which appears to be abundant in mouse oocytes. However, phylogenetic analysis of the entire sequence has revealed that Nox-U1 is closer to Nox4 or Nox5 than Nox2 or Nox3. Histidine residues assumed to be responsible for heme ligation, motifs for FAD- and NADPH-binding, and two asparagine-linked glycosylation sites are conserved.

MeSH Terms
Amino Acid Motifs Amino Acid Sequence Animals Base Sequence DNA Primers/chemistry DNA, Complementary/metabolism Female Gene Expression Regulation Heme/chemistry Histidine/chemistry Membrane Glycoproteins/biosynthesis Membrane Proteins/biosynthesis Mice Molecular Sequence Data NADH, NADPH Oxidoreductases/biosynthesis NADPH Oxidase 1 NADPH Oxidase 2 NADPH Oxidase 4 NADPH Oxidase 5 NADPH Oxidases/biosynthesis Oocytes Phylogeny RNA/metabolism Rats Sea Urchins Software Species Specificity Superoxides/metabolism Tissue Distribution
Chemicals
DNA Primers DNA, Complementary Membrane Glycoproteins Membrane Proteins Superoxides Heme Histidine RNA NADH, NADPH Oxidoreductases Cybb protein, mouse NADPH Oxidase 1 NADPH Oxidase 2 NADPH Oxidase 4 NADPH Oxidase 5 NADPH Oxidases NOX1 protein, mouse NOX1 protein, rat NOX5 protein, human Nox3 protein, mouse Nox4 protein, mouse
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maru Yoshiro
Department of Pharmacology, Tokyo Women's Medical University, Tokyo, Japan. [email protected]
Nishino Takeshi
Kakinuma Katsuko
Article Info
Journal
DNA sequence : the journal of DNA sequencing and mapping
Abbr.
DNA Seq
ISSN
1042-5179
DOI
10.1080/10425170500069734
Email
[email protected]
Published
2005-04-00
Pages
83-8
Language
English
Region
England
NLM ID
9107800
Subset
IM
External Links
PubMed source DOI: 10.1080/10425170500069734
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