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PMID: 1620105 Published · ppublish English Journal Article

High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.

Molecular and cellular biology ·Vol. 12 ·No. 7 ·1992-07-00 ·Pages 2919-23

Thomas KR, Deng C, Capecchi MR

Abstract

Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides by several kilobases of DNA homologous to the target locus, replacement of chromosomal sequences with the exogenous DNA occurred with precision. If, on the other hand, the target-homologous DNA on one arm of the vector was reduced to below 1 kb in length, the fidelity of recombination was diminished.

Related Genes
Hprt
MeSH Terms
Animals Drug Resistance Gene Rearrangement/genetics Genetic Vectors/genetics Gentamicins/pharmacology Mice Mutagenesis, Site-Directed/genetics Recombination, Genetic/genetics Selection, Genetic Stem Cells/metabolism Thioguanine/pharmacology Transformation, Genetic
Chemicals
Gentamicins antibiotic G 418 Thioguanine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thomas K R
HHMI Research Laboratories, Eccles Institute of Human Genetics, University of Utah Medical Center, Salt Lake City 84112.
Deng C
Capecchi M R
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25 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.7.2919-2923.1992
Published
1992-07-00
Pages
2919-23
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364504
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.12.7.2919-2923.1992 PMC Full Text View journal details
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