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PMID: 16246725 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

A Requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation.

Molecular cell ·Vol. 20 ·No. 2 ·2005-10-28 ·Pages 225-36

Sheldon KE, Mauger DM, Arndt KM

Abstract

RNA synthesis and processing are coordinated by proteins that associate with RNA polymerase II (pol II) during transcription elongation. The yeast Paf1 complex interacts with RNA pol II and mediates histone modifications during elongation. To elucidate the functions of this complex, we isolated missense mutations in the gene encoding the Rtf1 subunit and used them to identify functionally interacting proteins. We identified NAB3 as a dosage suppressor of rtf1. Nab3, together with Nrd1, directs 3' end formation of nonpolyadenylated RNA pol II transcripts, such as snoRNAs. Deletion of Paf1, but not the Set1, Set2, or Dot1 histone methyltransferases, causes accumulation of snoRNA transcripts that are extended at their 3' ends. The Paf1 complex associates with and facilitates Nrd1 recruitment to the SNR47 gene, suggesting a direct involvement in 3' end formation. Our results reveal a posttranscriptional function for the Paf1 complex, which appears unrelated to its role in histone methylation.

MeSH Terms
Gene Expression Regulation Mutation Nuclear Proteins/genetics,metabolism RNA 3' End Processing/physiology RNA Polymerase II/metabolism RNA, Messenger/genetics,metabolism RNA, Small Nucleolar/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Ribonucleoproteins/genetics,metabolism Saccharomyces cerevisiae/enzymology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism TATA-Box Binding Protein/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
NAB3 protein, S cerevisiae NRD1 protein, S cerevisiae Nuclear Proteins PAF1 protein, S cerevisiae RNA, Messenger RNA, Small Nucleolar RNA-Binding Proteins RTF1 protein, S cerevisiae Ribonucleoproteins Saccharomyces cerevisiae Proteins TATA-Box Binding Protein Transcription Factors RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sheldon Kathryn E
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
Mauger David M
Arndt Karen M
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-2765
DOI
10.1016/j.molcel.2005.08.026
Published
2005-10-28
Pages
225-36
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC1839845
Subset
IM
Grants
NIAID NIH HHS · K02 AI001816 · United States
NIGMS NIH HHS · R01 GM052593 · United States
NIAID NIH HHS · AI01816 · United States
NIGMS NIH HHS · GM52593 · United States
Corrections
CommentIn
PMID: 16246718
External Links
PubMed source DOI: 10.1016/j.molcel.2005.08.026 PMC Full Text View journal details
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