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PMID: 16264190 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF.

Genes & development ·Vol. 19 ·No. 21 ·2005-11-01 ·Pages 2534-9

Prochasson P, Florens L, Swanson SK, Washburn MP, Workman JL

Abstract

The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 proteins form a stable HIR repressor complex. The HIR complex promotes histone deposition onto DNA in vitro and constitutes a novel nucleosome assembly complex. The HIR complex stably binds to DNA and nucleosomes. Furthermore, HIR complex binding to nucleosomes forms a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Thus, the HIR complex is a novel nucleosome assembly complex which functions with SWI/SNF to regulate transcription.

MeSH Terms
DNA, Fungal/genetics,metabolism Gene Expression Regulation, Fungal/physiology Histones/biosynthesis,genetics Multiprotein Complexes/genetics,metabolism Nucleosomes/metabolism Repressor Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
DNA, Fungal Histones Multiprotein Complexes Nucleosomes Repressor Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Prochasson Philippe
The Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
Florens Laurence
Swanson Selene K
Washburn Michael P
Workman Jerry L
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1341105
Published
2005-11-01
Pages
2534-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1276727
Subset
IM
Grants
NIGMS NIH HHS · R37 GM047867 · United States
NIGMS NIH HHS · R37GM047867 · United States
External Links
PubMed source DOI: 10.1101/gad.1341105 PMC Full Text View journal details
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