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PMID: 1627150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of two types of homologous DNA pairing activity in mouse cells.

Biochemical and biophysical research communications ·Vol. 185 ·No. 3 ·1992-06-30 ·Pages 987-92

Mishina Y, Shimokawa T, Kawasaki K, Arai N, Shibata T, Koyama H

Abstract

We have identified two types of homologous DNA pairing activity in mouse cell extracts by a strand-transfer assay. Both activities are separated from each other by anion-exchange chromatography; neither of them needs ATP. One requires magnesium ion and is stimulated by Escherichia coli single-stranded DNA binding protein, whereas the other does not require the ion and shows a higher affinity for a left-handed Z-DNA.

MeSH Terms

Adenosine Triphosphate/metabolism Animals Chromatography, Affinity Chromatography, Ion Exchange Clone Cells DNA, Bacterial/genetics,metabolism DNA, Circular/genetics,metabolism DNA, Single-Stranded/genetics,metabolism DNA-Binding Proteins/metabolism Escherichia coli/metabolism Magnesium/metabolism Mammary Neoplasms, Experimental Mice Substrate Specificity Tumor Cells, Cultured

Chemicals

DNA, Bacterial DNA, Circular DNA, Single-Stranded DNA-Binding Proteins Adenosine Triphosphate Magnesium

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Mishina Y

Kihara Institute for Biological Research, Yokohama City University, Japan.

Shimokawa T

Kawasaki K

Arai N

Shibata T

Koyama H

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(92)91724-5

Published

1992-06-30

Pages

987-92

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1016/0006-291x(92)91724-5 View journal details

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Identification of two types of homologous DNA pairing activity in mouse cells.

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