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PMID: 16278345 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Interactions between the cell cycle and embryonic patterning in Arabidopsis uncovered by a mutation in DNA polymerase epsilon.

The Plant cell ·Vol. 17 ·No. 12 ·2005-12-00 ·Pages 3362-77

Jenik PD, Jurkuta RE, Barton MK

Abstract

Pattern formation and morphogenesis require coordination of cell division rates and orientations with developmental signals that specify cell fate. A viable mutation in the TILTED1 locus, which encodes the catalytic subunit of DNA polymerase epsilon of Arabidopsis thaliana, causes a lengthening of the cell cycle by approximately 35% throughout embryo development and alters cell type patterning of the hypophyseal lineage in the root, leading to a displacement of the root pole from its normal position on top of the suspensor. Treatment of preglobular and early globular stages, but not later stage, embryos with the DNA polymerase inhibitor aphidicolin leads to a similar phenotype. The results uncover an interaction between the cell cycle and the processes that determine cell fate during plant embryogenesis.

MeSH Terms
Amino Acid Sequence Arabidopsis/cytology,embryology,enzymology Base Sequence Body Patterning Catalysis Cell Cycle Cloning, Molecular DNA Polymerase II/chemistry,genetics DNA Primers DNA, Plant/metabolism Molecular Sequence Data Mutation Reverse Transcriptase Polymerase Chain Reaction Seeds/growth & development,metabolism Sequence Homology, Amino Acid
Chemicals
DNA Primers DNA, Plant DNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jenik Pablo D
Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA.
Jurkuta Rebecca E J
Barton M Kathryn
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.036889
Published
2005-12-00
Epub
2005-00-08
Pages
3362-77
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1315375
Subset
IM
Databases
GENBANK
AAH63246, AAP12650, P21951
RefSeq
NP_172303, NP_180280, NP_493616, NP_524462, NP_596354, XP_465943
External Links
PubMed source DOI: 10.1105/tpc.105.036889 PMC Full Text View journal details
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