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PMID: 1629331 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction.

Journal of clinical microbiology ·Vol. 30 ·No. 7 ·1992-07-00 ·Pages 1752-7

Menzo S, Bagnarelli P, Giacca M, Manzin A, Varaldo PE, Clementi M

Abstract

A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human immunodeficiency virus type 1 (HIV-1) viremia was developed and optimized. This method consists of the reverse transcription and subsequent amplification in the same tube of two similar RNA templates, the wild-type template to be quantified and a known amount of the internally deleted synthetic template, both with identical primer recognition sites. The same strategy also proved to be useful in the quantitative assay of HIV-1-specific cellular transcripts and proviral DNA sequences from peripheral blood mononuclear cells by using competitor DNA. The method might be of interest in the study of the precise level of HIV-1 activity during the different clinical phases of the infection and in the simple, fast, and methodologically correct molecular investigation of patients treated with specific antiviral compounds.

MeSH Terms
Base Sequence HIV Infections/microbiology HIV-1/isolation & purification Humans Molecular Sequence Data Polymerase Chain Reaction/methods Transcription, Genetic Viremia/diagnosis
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Menzo S
Institute of Microbiology, University of Ancona, Italy.
Bagnarelli P
Giacca M
Manzin A
Varaldo P E
Clementi M
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.7.1752-1757.1992
Published
1992-07-00
Pages
1752-7
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265375
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.7.1752-1757.1992 PMC Full Text View journal details
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