Recovery of murine leukemia virus from large volumes of freshly harvested culture fluids by using a single density gradient.

Applied microbiology ·Vol. 29 ·No. 1 ·1975-01-00 ·Pages 102-5

Sottong P, Hill P, Feeney M, Klecker J, Johnson K, Harris R, Bell C, Stafford K

Abstract

Concentration and purification of murine leukemia virus for use as complement fixation antigens was accomplished by using a single density gradient in the model RK ultracentrifuge.

MeSH Terms

Animals Antigens, Viral Cell Line Centrifugation, Density Gradient Complement Fixation Tests/standards Formaldehyde Goats/immunology Immune Sera Leukemia Virus, Murine/isolation & purification,ultrastructure Mice/immunology Mice, Inbred Strains Microscopy, Electron Rats/immunology Spectrophotometry, Ultraviolet Sucrose Surface-Active Agents Viral Proteins

Chemicals

Antigens, Viral Immune Sera Surface-Active Agents Viral Proteins Formaldehyde Sucrose

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Sottong P

Hill P

Feeney M

Klecker J

Johnson K

Harris R

Bell C

Stafford K

References (10)

10 references, click to expand

Large-volume purification of tumor viruses by use of zonal centrifuges.
Appl Microbiol. 1972 May;23(5):1010-4 PMID: 4113253
Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture.
J Virol. 1969 Feb;3(2):126-32 PMID: 4304442
Production of altered cell foci by 3-methylcholanthrene in mouse cells infected with AKR leukemia virus.
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2212-6 PMID: 4332249
Immunological relationships of reverse transcriptases from ribonucleic acid tumor viruses.
J Virol. 1972 Jan;9(1):110-5 PMID: 4333537
Production and purification of large amounts of Rous sarcoma virus.
Appl Microbiol. 1973 Mar;25(3):346-53 PMID: 4349185
Large-scale purification of ribonucleic acid tumor viruses by use of continuous-flow density gradient centrifugation.
Appl Microbiol. 1973 Sep;26(3):452-4 PMID: 4356466
K-series centrifuges. I. Development of the K-II continuous-sample-flow-with-banding centrifuge system for vaccine purification.
Anal Biochem. 1969 Dec;32(3):460-94 PMID: 5361400
Application of a microtechnique to viral serological investigations.
J Immunol. 1962 Mar;88:320-9 PMID: 13910995
Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.
Appl Microbiol. 1970 Aug;20(2):259-62 PMID: 4098102
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.29.1.102-105.1975

Published

1975-01-00

Pages

102-5

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC186918

Subset

External Links

PubMed source DOI: 10.1128/am.29.1.102-105.1975 PMC Full Text

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Recovery of murine leukemia virus from large volumes of freshly harvested culture fluids by using a single density gradient.

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