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PMID: 16299223 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sugars and circadian regulation make major contributions to the global regulation of diurnal gene expression in Arabidopsis.

The Plant cell ·Vol. 17 ·No. 12 ·2005-12-00 ·Pages 3257-81

Bläsing OE, Gibon Y, Günther M, Höhne M, Morcuende R, Osuna D, Thimm O, Usadel B, Scheible WR, Stitt M

Abstract

The diurnal cycle strongly influences many plant metabolic and physiological processes. Arabidopsis thaliana rosettes were harvested six times during 12-h-light/12-h-dark treatments to investigate changes in gene expression using ATH1 arrays. Diagnostic gene sets were identified from published or in-house expression profiles of the response to light, sugar, nitrogen, and water deficit in seedlings and 4 h of darkness or illumination at ambient or compensation point [CO(2)]. Many sugar-responsive genes showed large diurnal expression changes, whose timing matched that of the diurnal changes of sugars. A set of circadian-regulated genes also showed large diurnal changes in expression. Comparison of published results from a free-running cycle with the diurnal changes in Columbia-0 (Col-0) and the starchless phosphoglucomutase (pgm) mutant indicated that sugars modify the expression of up to half of the clock-regulated genes. Principle component analysis identified genes that make large contributions to diurnal changes and confirmed that sugar and circadian regulation are the major inputs in Col-0 but that sugars dominate the response in pgm. Most of the changes in pgm are triggered by low sugar levels during the night rather than high levels in the light, highlighting the importance of responses to low sugar in diurnal gene regulation. We identified a set of candidate regulatory genes that show robust responses to alterations in sugar levels and change markedly during the diurnal cycle.

MeSH Terms
Arabidopsis/genetics Carbohydrates/physiology Circadian Rhythm Gene Expression Regulation, Plant/physiology Genes, Plant Mutation Nitrogen/physiology RNA, Messenger/genetics
Chemicals
Carbohydrates RNA, Messenger Nitrogen
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Bläsing Oliver E
Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany. [email protected]
Gibon Yves
Günther Manuela
Höhne Melanie
Morcuende Rosa
Osuna Daniel
Thimm Oliver
Usadel Björn
Scheible Wolf-Rüdiger
Stitt Mark
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.035261
Email
[email protected]
Published
2005-12-00
Epub
2005-00-18
Pages
3257-81
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1315368
Subset
IM
Databases
OMIM
GSE3416, GSE3423, GSE3424
External Links
PubMed source DOI: 10.1105/tpc.105.035261 PMC Full Text View journal details
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