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PMID: 16299479 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review

Fiber-optic fluorescence imaging.

Nature methods ·Vol. 2 ·No. 12 ·2005-12-00 ·Pages 941-50

Flusberg BA, Cocker ED, Piyawattanametha W, Jung JC, Cheung EL, Schnitzer MJ

Abstract

Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue. A challenge in the creation of such devices is the design and integration of miniaturized optical and mechanical components. Until recently, fiber-based fluorescence imaging was mainly limited to epifluorescence and scanning confocal modalities. Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging. An upcoming generation of fluorescence imaging devices will be based on microfabricated device components.

MeSH Terms
Animals Fiber Optic Technology/methods Humans Microscopy, Fluorescence/methods Optical Fibers
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Flusberg Benjamin A
James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, 318 Campus Drive, Stanford, California 94305, USA.
Cocker Eric D
Piyawattanametha Wibool
Jung Juergen C
Cheung Eunice L M
Schnitzer Mark J
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7091
DOI
10.1038/nmeth820
Published
2005-12-00
Pages
941-50
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC2849801
Subset
IM
Grants
NINDS NIH HHS · R01 NS050533 · United States
NINDS NIH HHS · R01 NS050533-01 · United States
NIDA NIH HHS · R21 DA017895 · United States
NIDA NIH HHS · R21 DA017895-01 · United States
External Links
PubMed source DOI: 10.1038/nmeth820 PMC Full Text View journal details
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