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PMID: 163224 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Novel pathway for degradation of protocatechuic acid in Bacillus species.

Journal of bacteriology ·Vol. 121 ·No. 2 ·1975-02-00 ·Pages 531-6

Crawford RL

Abstract

A species of Bacillus, tentatively identified as B. circulans, degrades protocatechuic acid by a novel reaction involving meta-fission between C2 and C3 of the benzene nucleus. 2-Hydroxymuconic semialdehyde is then degraded to pyruvate and acetaldehyde by enzymatic reactions described in previous work. Protocatechuate 2,3-oxygenase exhibits a rather narrow substrate specificity; the methyl and ethyl esters of protocatechuic acid are oxidized, but other substrates for ring-fission oxygenases, notably catechol, gallic acid, and homoprotocatechuic acid, are not attached.

MeSH Terms

Acetaldehyde/biosynthesis Aldehydes/biosynthesis Bacillus/enzymology,metabolism Benzoates/metabolism Catechols/metabolism Cell-Free System Chemical Phenomena Chemistry Fatty Acids, Unsaturated/biosynthesis Fatty Alcohols/biosynthesis Hydrolases/metabolism NAD/metabolism Oxidoreductases/metabolism Oxygen Consumption Oxygenases/metabolism Pseudomonas/metabolism Pyruvates/biosynthesis Spectrophotometry

Chemicals

Aldehydes Benzoates Catechols Fatty Acids, Unsaturated Fatty Alcohols Pyruvates NAD Oxidoreductases Oxygenases Hydrolases Acetaldehyde

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Crawford R L

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.121.2.531-536.1975

Published

1975-02-00

Pages

531-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245963

Subset

External Links

PubMed source DOI: 10.1128/jb.121.2.531-536.1975 PMC Full Text View journal details

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Novel pathway for degradation of protocatechuic acid in Bacillus species.

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