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PMID: 16322557 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.

Genes & development ·Vol. 19 ·No. 23 ·2005-12-01 ·Pages 2816-26

Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky EM, Snyder M, Grayhack EJ

Abstract

Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control. Analysis of 5573 MORFs demonstrates that nearly all verified ORFs are expressed, suggests the authenticity of 48 ORFs characterized as dubious, and implicates specific processes including cytoskeletal organization and transcriptional control in growth inhibition caused by overexpression. Global analysis of glycosylated proteins identifies 109 new confirmed N-linked and 345 candidate glycoproteins, nearly doubling the known yeast glycome.

MeSH Terms
Cell Growth Processes/genetics Fungal Proteins/analysis,genetics Gene Library Glycoproteins/analysis,genetics Open Reading Frames Proteome Proteomics/methods Yeasts/genetics
Chemicals
Fungal Proteins Glycoproteins Proteome
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Gelperin Daniel M
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
White Michael A
Wilkinson Martha L
Kon Yoshiko
Kung Li A
Wise Kevin J
Lopez-Hoyo Nelson
Jiang Lixia
Piccirillo Stacy
Yu Haiyuan
Gerstein Mark
Dumont Mark E
Phizicky Eric M
Snyder Michael
Grayhack Elizabeth J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1362105
Published
2005-12-01
Pages
2816-26
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1315389
Subset
IM
Grants
NIGMS NIH HHS · R01 GM036494 · United States
NCI NIH HHS · R01 CA077808 · United States
NHGRI NIH HHS · R01 HG002311 · United States
NIGMS NIH HHS · GM62480-04 · United States
NHGRI NIH HHS · HG02311 · United States
NIGMS NIH HHS · GM36494-17 · United States
NIGMS NIH HHS · R01 GM062480 · United States
NCI NIH HHS · CA77808-09 · United States
External Links
PubMed source DOI: 10.1101/gad.1362105 PMC Full Text View journal details
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