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PMID: 163352 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Alteration of capsid proteins of coxsackievirus A13 by low ionic concentrations.

Journal of virology ·Vol. 15 ·No. 2 ·1975-02-00 ·Pages 244-52

Cords CE, James CG, McLaren LC

Abstract

Several group A coxsackieviruses (A13, 15, 18, and 21), but not polioviruses or group B coxsackieviruses, are rapidly inactivated in low ionic strength solutions at neutral pH. The extent of inactivation is dependent upon temperature and molarity. Virions inactivated in this manner contain a normal complement of infectious RNA which remains in a state resistant to the action of ribonuclease. However, more than 95% of the virus particles are unable to attach to susceptible cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals that coxsackievirus A13 virions contain five structural polypeptides (VP1, VP2a, VP2b, VP3, and VP4). Electrophoretic analysis indicates that inactivation of coxsackievirus A13 in low ionic strength solutions is due to the specific loss of the smallest polypeptide VP4 from the virus particle. These results suggest that adsorption of coxsackievirus A13 to receptors on susceptible cells is dependent upon the presence of the capsid protein VP4.

MeSH Terms
Adsorption Amino Acids/metabolism Buffers Carbon Radioisotopes Electrophoresis, Polyacrylamide Gel Enterovirus/drug effects,growth & development,metabolism HeLa Cells Humans Hypotonic Solutions Peptides/metabolism Poliovirus/drug effects,growth & development Temperature Tritium Uridine/metabolism Viral Plaque Assay Viral Proteins/metabolism
Chemicals
Amino Acids Buffers Carbon Radioisotopes Hypotonic Solutions Peptides Viral Proteins Tritium Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cords C E
James C G
McLaren L C
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20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.2.244-252.1975
Published
1975-02-00
Pages
244-52
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354446
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.2.244-252.1975 PMC Full Text View journal details
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