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PMID: 163361 Published · ppublish English Journal Article

Inhibition of bacteriophage PBS2 replication in Bacillus subtilis by phleomycin.

Journal of virology ·Vol. 15 ·No. 2 ·1975-02-00 ·Pages 363-71

Post L, Price AR

Abstract

Phleomycin is an effective inhibitor of the replication of Bacillus subtilis bacteriophage PBS2, whose DNA contains uracil instead of thymine. Phleomycin does not affect the induction of the known phage enzymes involved in deoxyribonucleotide metabolism. But phage DNA synthesis is severely inhibited by phleomycin, and late virion protein synthesis is eliminated. These effects appear to result from a phleomycin-induced degradation of the parental phage DNA. Similar inhibitory and degradative effects on DNA are seen in phleomyinc-treated, uninfected cells. This system is unaffected by the related antibiotic, bleomycin.

MeSH Terms
Adenine/metabolism Amino Acids/metabolism Aminohydrolases/biosynthesis Antibiotics, Antineoplastic/pharmacology Bacillus subtilis Bacteriophages/drug effects,growth & development,metabolism Carbon Radioisotopes DNA Nucleotidyltransferases/biosynthesis DNA Viruses DNA, Viral/biosynthesis,metabolism Peptides Phosphoric Monoester Hydrolases/biosynthesis Phosphorus Radioisotopes Pyrophosphatases/biosynthesis Tritium Viral Proteins/biosynthesis Virus Replication/drug effects
Chemicals
Amino Acids Antibiotics, Antineoplastic Carbon Radioisotopes DNA, Viral Peptides Phosphorus Radioisotopes Viral Proteins Tritium DNA Nucleotidyltransferases Phosphoric Monoester Hydrolases Aminohydrolases Pyrophosphatases Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Post L
Price A R
References (29)
29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.2.363-371.1975
Published
1975-02-00
Pages
363-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354461
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.2.363-371.1975 PMC Full Text View journal details
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