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PMID: 16344550 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Differential use of functional domains by coiled-coil coactivator in its synergistic coactivator function with beta-catenin or GRIP1.

The Journal of biological chemistry ·Vol. 281 ·No. 6 ·2006-02-10 ·Pages 3389-97

Yang CK, Kim JH, Li H, Stallcup MR

Abstract

beta-Catenin, a pivotal component of the Wnt-signaling pathway, binds to and serves as a transcriptional coactivator for the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcriptional activator proteins and for the androgen receptor (AR), a nuclear receptor. Three components of the p160 nuclear receptor coactivator complex, including CARM1, p300/CBP, and GRIP1 (one of the p160 coactivators), bind to and cooperate with beta-catenin to enhance transcriptional activation by TCF/LEF and AR. Here we report that another component of the p160 nuclear receptor coactivator complex, the coiled-coil coactivator (CoCoA), directly binds to and cooperates synergistically with beta-catenin as a coactivator for AR and TCF/LEF. CoCoA uses different domains to bind GRIP1 and beta-catenin, and it uses different domains to transmit the activating signal to the transcription machinery, depending on whether it is bound to GRIP1 or beta-catenin. CoCoA associated specifically with the promoters of transiently transfected and endogenous target genes of TCF/LEF, and reduction of the endogenous CoCoA level decreased the ability of TCF/LEF and beta-catenin to activate transcription of transient and endogenous target genes. Thus, CoCoA uses different combinations of functional domains to serve as a physiologically relevant component of the Wnt/beta-catenin signaling pathway and the androgen signaling pathway.

MeSH Terms
Animals COS Cells Calcium-Binding Proteins Carrier Proteins/chemistry,metabolism Cell Line Cell Line, Tumor Chickens Chlorocebus aethiops Chromatin Immunoprecipitation Enhancer Elements, Genetic Glutathione Transferase/metabolism Humans Immunoprecipitation Lymphoid Enhancer-Binding Factor 1/metabolism Models, Biological Nerve Tissue Proteins/chemistry,metabolism Plasmids/metabolism Promoter Regions, Genetic Protein Binding Protein Interaction Mapping Protein Structure, Tertiary RNA Interference Signal Transduction TCF Transcription Factors/metabolism Transcription Factors Transcription, Genetic Transcriptional Activation Transfection beta Catenin/metabolism,physiology
Chemicals
CALCOCO1 protein, human Calcium-Binding Proteins Carrier Proteins GRIP1 protein, human Lymphoid Enhancer-Binding Factor 1 Nerve Tissue Proteins TCF Transcription Factors Transcription Factors beta Catenin Glutathione Transferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang Catherine K
Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles, California 90089, USA.
Kim Jeong Hoon
Li Hongwei
Stallcup Michael R
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
DOI
10.1074/jbc.M510403200
Published
2006-02-10
Epub
2005-00-12
Pages
3389-97
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC1626527
Subset
IM
Grants
NIDDK NIH HHS · R01 DK043093 · United States
NIDDK NIH HHS · DK43093 · United States
External Links
PubMed source DOI: 10.1074/jbc.M510403200 PMC Full Text View journal details
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